MODIFIED RNA SEQUENCE POOLS FOR IN-VITRO SELECTION
MODIFIED RNA SEQUENCE POOLS FOR IN-VITRO SELECTION
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DOI：
10.1093/nar/22.24.5229
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发表时间：
1994-12-11
影响因子：
14.9
通讯作者：
JAYASENA, SD
中科院分区：
文献类型：
--
作者：
LIN, Y;QIU, Q;JAYASENA, SD
我们报告了使用修饰的RNA，其中嘧啶的2 '-OH基团被2'-氨基（2 '-NH 2）基团取代，以通过体外选择鉴定对人中性粒细胞弹性蛋白酶（HNE）特异的高亲和力配体。与未修饰的RNA相比，P '-NH 2修饰的RNA配体在人血清和尿中显示出增强的稳定性。在K+和Li+离子存在下使用RNase T1切割数据表明，为HNE选择的修饰的RNA配体形成分子间G-四重结构。
We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the P'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.