Identification of a novel receptor mediating substance P-induced behavior in the mouse.
Identification of a novel receptor mediating substance P-induced behavior in the mouse.
复制标题
鉴定介导 P 物质诱导的小鼠行为的新型受体。
DOI：
10.1016/0014-2999(92)90849-y
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发表时间：
1992
影响因子：
5
通讯作者：
Larson,AA
中科院分区：
文献类型：
--
作者：
Mousseau,DD;Sun,X;Larson,AA
为了确定阿片受体或最近表征的纳洛酮敏感物质P(SP)N-末端结合位点是否在SP的行为效应脱敏中起作用，我们评估了选择性拮抗剂对μ-(纳洛酮和？)、δ-(纳曲哚)和κ-(去甲肾上腺素)阿片受体的作用，以及[~3H]-SP-(1-7)的抑制剂[D-Pro2，D-Leu7]SP-(1-7)D-SP(1-7)的作用鞘内注射SP对小鼠行为学的影响非选择性阿片受体拮抗剂纳洛酮可抑制对SP的行为脱敏，但选择性阿片受体拮抗剂对重复给药的反应无明显影响。然而，与纳洛酮一样，SP-(1-7)拮抗剂可抑制SP诱导的脱敏。D-SP-(1-7)对SP脱敏的保护作用，而不是μ，δ或κ受体的选择性拮抗剂，表明对SP的行为效应的脱敏似乎不是通过阿片受体的作用而是通过SP-(1-7)结合部位的作用来实现的。
To determine whether opioid receptors or the more recently characterized naloxone-sensitive substance P (SP) N-terminal binding sites play a role in desensitization to the behavioral effects of SP, we assessed the effects of selective antagonists at μ- (naloxonazine and ß-funaltrexamine), δ- (naltrindole) and κ- (nor-binaltorphimine) opioid receptors, as well as the effect of [D-Pro2,D-Leu7]SP-(1-7) D-SP-(1-7) (D-SP (1-7)), an inhibitor of [3H]SP-(1-7) binding, on behaviors induced by intrathecally administered SP in mice. Whereas naloxone, a non-selective opioid antagonist, inhibited the development of behavioral desensitization to SP, the response to repeated SP administration remained unaffected by pretreatment with selective opioid antagonists. Like naloxone, however, the SP-(1-7) antagonist inhibited SP-induced desensitization. The protection against desensitization to SP by D-SP-(1-7), but not by selective antagonists of μ, δ or κ receptors, suggests that desensitization to the behavioral effects of SP does not appear to be mediated by an action at an opioid receptor but by an action at the SP-(1-7) binding site.