Heat Tolerance in Diploid Wild Potato Species In Vitro
Heat Tolerance in Diploid Wild Potato Species In Vitro
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DOI：
10.1007/s12230-019-09716-9
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发表时间：
2019-06-01
影响因子：
1.5
通讯作者：
Pinto, Cesar A. B. P.
中科院分区：
文献类型：
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作者：
Guedes, Marcio L.;Haynes, Kathleen G.;Pinto, Cesar A. B. P.
马铃薯是一种凉季作物。然而，随着世界人口的增长和气温的上升，将需要更多的耐热品种。这项工作的目的是确定耐热性二倍体野生茄属物种，作为衡量的能力，形成微型块茎的组织培养，并比较四种不同的方法来评估耐热性。在随时间进行的五个实验中的每一个中，将来自10个二倍体野生马铃薯物种中的每一个的多达20个基因型的四个节插条在补充有8%蔗糖的MS培养基中生长，并在黑暗中在19 ° C（冷）和25 ° C（暖）下培养。用四种方法来评价耐热性：在温暖条件下的微体结薯率，在凉爽和温暖条件下的微体结薯率的差异，在温暖条件下的微体结薯产量，以及在凉爽和温暖条件下的微体结薯产量的差异。对于这四种方法中的每一种，对物种进行排名（排名=1是最耐热的），并对四种方法的排名进行求和，以确定最耐热的方法。种间差异显著，种×温度互作对试管薯率和试管薯产量影响显著。在五个物种的微体化百分比是相似的凉爽和温暖的温度下，更大的三个物种的凉爽的温度下，和，更大的温暖的温度下的两个物种。在四个物种的微型薯产量是相似的凉爽和温暖的温度下，更大的凉爽温度下的六个物种，和，更大的温暖温度下的一个物种。不同种的耐热性排序因所用方法而异，但S。kurtzianum和S. sogarandinum最耐热。
Potatoes are a cool season crop. Yet, with growth in the world population and rising temperatures, more heat tolerant cultivars are going to be needed. The objectives of this work were to identify heat tolerance in diploid wild Solanum species, as measured by the ability to form microtubers in tissue culture, and to compare four different methods of evaluating heat tolerance. In each of five experiments conducted over time, four nodal cuttings from up to 20 genotypes from each of ten diploid wild potato species were grown in MS medium supplemented with 8% sucrose and cultured at 19 degrees C (cool) and 25 degrees C (warm) in the dark. Four methods were used to evaluate heat tolerance: percent microtuberization under warm conditions, difference in percent microtuberization between cool and warm conditions, microtuber yield under warm conditions, and the difference in microtuber yield between cool and warm conditions. Species were ranked for each of these four methods (with rank=1 being most heat tolerant) and ranks were summed across the four methods to identify the most heat tolerant. There were significant differences among species and the species x temperature interaction was significant for percent microtuberization and microtuber yield. Percent microtuberization in five of the species was similar under cool and warm temperatures; greater under cool temperatures for three species; and, greater under warm temperatures for two species. Microtuber yield in four of the species was similar under cool and warm temperatures; greater under cool temperatures for six species; and, greater under warm temperatures for one species. Rankings of heat tolerance among the species varied by the method used, but S. kurtzianum and S. sogarandinum were the most heat tolerant.