Mutations affecting development of the notochord in zebrafish.
Mutations affecting development of the notochord in zebrafish.
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DOI：
10.5167/uzh-238
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发表时间：
1996-12
期刊：
影响因子：
4.6
通讯作者：
D. Stemple;L. Solnica-Krezel;F. Zwartkruis;S. Neuhauss;A. Schier;J. Malicki;D. Stainier;S. Abdelilah;Z. Rangini;E. Mountcastle-Shah;W. Driever
中科院分区：
文献类型：
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作者：
D. Stemple;L. Solnica-Krezel;F. Zwartkruis;S. Neuhauss;A. Schier;J. Malicki;D. Stainier;S. Abdelilah;Z. Rangini;E. Mountcastle-Shah;W. Driever
脊索对于脊椎动物胚胎的正常发育至关重要。它既是胚胎的主要骨骼元素，也是神经外胚层、近轴中胚层和其他组织内模式建立的信号源。在一个大规模的系统筛选突变影响胚胎发生在斑马鱼，我们确定了65个突变，属于29个互补组，每个导致的缺陷，在形成和/或维护脊索。这些突变在脊索发育的许多阶段产生表型异常，从而建立表型途径，这反过来又表明脊索发育的遗传途径。突变胚胎中相邻组织内的扰动进一步表明了脊索来源的信号对胚胎内图案化的重要性，并表明这些突变将产生对调节这些图案化过程的线索的额外了解。
The notochord is critical for the normal development of vertebrate embryos. It serves both as the major skeletal element of the embryo and as a signaling source for the establishment of pattern within the neurectoderm, the paraxial mesoderm and other tissues. In a large-scale systematic screen of mutations affecting embryogenesis in zebrafish we identified 65 mutations that fall into 29 complementation groups, each leading to a defect in the formation and/or maintenance of the notochord. These mutations produce phenotypic abnormalities at numerous stages of notochord development, thereby establishing a phenotypic pathway, which in turn suggests a genetic pathway for the development of the notochord. Perturbations within adjacent tissues in mutant embryos further indicate the importance of notochord-derived signals for patterning within the embryo and suggest that these mutations will yield additional insight into the cues that regulate these patterning processes.