Expression of Dnmt3b in mouse hematopoietic progenitor cells and spermatogonia at specific stages
Expression of Dnmt3b in mouse hematopoietic progenitor cells and spermatogonia at specific stages
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DOI：
10.1016/j.modgep.2004.06.008
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发表时间：
2004-11-01
影响因子：
1.2
通讯作者：
Hanaoka, K
中科院分区：
文献类型：
--
作者：
Watanabe, D;Suetake, I;Hanaoka, K
两种新生型DNA甲基转移酶Dnmt3a和Dnmt3b负责在发育过程中创建DNA甲基化模式。Dnmt3b在小鼠早期胚胎的全能细胞中特异性表达，而Dnmt3a（两种同种型的较长形式）在10天胚胎期后的间充质细胞中普遍表达[Mech.Dev. 118（2002）187]。在本研究中，我们证明，Dnmt 3b在特定细胞的核中表达在某些组织中的10天胚胎阶段后。在胎肝、背主动脉和门静脉中，Dnmt 3b在表达CD 34的细胞中表达，表明这些细胞是造血祖细胞。Dnmt 3b在胚胎8天卵黄囊血岛造血祖细胞和成体骨髓细胞中均不表达。Dnmt 3b在出生后的A型精原细胞中也有表达。dnmt 3b不仅在全能干细胞中表达，而且在分化方向已经确定的祖细胞中也表达。另一方面，Dnmt 3a的长型在胎肝或A型精原细胞的这些造血祖细胞中不表达，但在胎肝和B型精原细胞的肝细胞中表达。Dnmt3b在异染色质和常染色质区域均有分布，而Dnmt3a则特异性定位于常染色质区域。(C)2004 Elsevier B.V.保留所有权利。
Two de novo-type DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation of DNA methylation patterns during development. Dnmt3b is specifically expressed in the totipotent cells of mouse early embryos and Dnmt3a, a longer form of the two isoforms, is ubiquitously expressed in mesenchyme cells after the 10 day embryo stage [Mech. Dev. 118 (2002) 187]. In the present study, we demonstrated that Dnmt3b was expressed in the nuclei of specific cells in certain tissues after the 10 day embryo stage. In fetal liver, dorsal aorta and portal vein, Dnmt3b was expressed in cells expressing CD34, indicating that the cells were hematopoietic progenitor cells. However, Dnmt3b was not expressed in the hematopoietic progenitor cells in yolk sac blood islands at 8 day embryo stage and in adult bone marrow cells. Dnmt3b was also expressed in type-A spermatogonia after birth. Dnmt3b was expressed not only in the totipotent stem cells but also in the progenitor cells the direction of differentiation of which had been already determined. On the other hand, the long form of Dnmt3a was not expressed in these hematopoietic progenitor cells in fetal liver or type-A spermatogonia, but was expressed in hepatocytes in fetal liver and type-B spermatogonia. While Dnmt3b was distributed in both the heterochromatin and euchromatin regions, Dnmt3a was specifically localized to the euchromatin region. (C) 2004 Elsevier B.V. All rights reserved.