Fluorometric determination of gizzerosine, a histamine H2-receptor agonist discovered in feedstuffs, employing high-performance liquid chromatography.
Fluorometric determination of gizzerosine, a histamine H2-receptor agonist discovered in feedstuffs, employing high-performance liquid chromatography.
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DOI：
10.1016/0003-2697(85)90048-x
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发表时间：
1985-11
影响因子：
2.9
通讯作者：
Y. Ito;T. Noguchi;H. Naito
中科院分区：
文献类型：
--
作者：
Y. Ito;T. Noguchi;H. Naito
在鱼粉中发现了2-氨基-9-（4-咪唑基）-7-氮杂壬酸，是雏鸡砂囊侵蚀的致病物质。该化合物作为组胺h2受体激动剂刺激胃酸分泌，诱导雏鸡砂囊糜烂。建立了高效液相色谱法测定该化合物含量的方法。该方法可测定鱼粉中0.2 ~ 5.0 μg的黄花苷含量，回收率为95 ~ 102%。导致雏鸡严重砂囊侵蚀的鱼粉含糖量超过20 mg/kg。该方法将有助于防止雏鸡砂囊侵蚀，这是一个大问题，在世界各地的家禽业。
Gizzerosine (2-amino-9-(4-imidazolyl)-7-azanonanoic acid) was discovered in fish meal as a causative material of gizzard erosion in chicks. This compound stimulates gastric acid secretion as a histamine H2-receptor agonist and induces gizzard erosion in chicks. A method of fluorometric determination of this compound was developed employing high-performance liquid chromatography. By this method, 0.2 to 5.0 μg of gizzerosine in fish meals was quantitated with the recovery of 95–102%. Fish meals, which induced severe gizzard erosion in chicks, contained more than 20 mg/kg of gizzerosine. The present method will be useful in preventing gizzard erosion in chicks, which is a big problem in the poultry industry all over the world.