Cooccurrence of Mycotoxins in Maize and Poultry Feeds from Brazil by Liquid Chromatography/Tandem Mass Spectrometry
Cooccurrence of Mycotoxins in Maize and Poultry Feeds from Brazil by Liquid Chromatography/Tandem Mass Spectrometry
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DOI：
10.1155/2013/427369
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Schuhmacher, Rainer
中科院分区：
文献类型：
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作者：
Mendes de Souza, Maria de Lourdes;Sulyok, Michael;Schuhmacher, Rainer
本研究的目的是定量评估巴西生产的玉米和家禽饲料样品中的真菌毒素。采用HPLC-MS/MS法对119份家禽饲料厂综合养殖场样品（玉米粒74份、家禽饲料36份、饲料厂废渣9份）中真菌代谢产物的检出情况进行了研究。在所研究的101种真菌代谢物中，有20种在样品中被检测和定量：黄曲霉毒素B-1、B-2、G（1）和G（2），伏马菌素B-1、B-2和B-3，水解伏马菌素B-1、玉米赤霉烯酮、农棒麦角碱、裸麦角碱、脱氧雪腐镰刀菌烯醇和雪腐镰刀菌烯醇，以及恩镰孢菌素A、A（1）、B、B-1、白僵菌素、曲酸和念珠藻素。大多数样品被一种以上的霉菌毒素污染。所有样品均被伏马菌素污染，玉米、饲料和工厂残留物样品的中位值分别为1，840 μ g/kg、239 μ g/kg和23，676 μ g/kg。令人惊讶的是，在超过90%的样品中检测到白僵菌素。黄曲霉毒素和二孢霉素的污染中值较低，接近LOD值。工厂残留物对所有真菌毒素的污染水平最高。这是第一次研究处理来自巴西的玉米和家禽饲料中的田麦角碱、裸麦角碱、恩镰孢菌素A、A（1）、B、B-1、白僵菌素和曲酸污染。
The objective of this study was to quantitatively evaluate mycotoxins in samples of maize and poultry feed produced in Brazil. A multimycotoxin method based on HPLC-MS/MS was applied to investigate the occurrence of toxical fungal metabolites in 119 samples collected from poultry feed factory integrated poultry farms: maize grain (74), poultry feed (36), and feed factory residue (9). Twenty of 101 fungal metabolites investigated were detected and quantified in the samples: aflatoxins B-1, B-2, G(1), and G(2), fumonisins B-1, B-2, and B-3, hydrolyzed fumonisin B-1, zearalenone, agroclavine, chanoclavine, deoxynivalenol, and nivalenol, and enniatin A, A(1), B, B-1, beauvericin, kojic acid, and moniliformin. Most samples were contaminated with more than one mycotoxin. All samples were contaminated with fumonisins, with medians values of 1,840 mu g/kg, 239 mu g/kg, and 23,676 mu g/kg for maize, feed, and factory residue samples, respectively. Surprisingly, beauvericin was detected in more than 90% of samples. The median contaminations of aflatoxin and trichothecenes were low, near LOD values. The factory residue presented highest contamination levels for all mycotoxins. This is the first study dealing with agroclavine, chanoclavine, enniatin A, A(1), B, B-1, beauvericin, and kojic acid contamination of maize and poultry feeds from Brazil.