BRAIN LEVELS OF NADH AND NAD+ UNDER HYPOXIC AND HYPOGLYCEMIC CONDITIONS INVITRO
BRAIN LEVELS OF NADH AND NAD+ UNDER HYPOXIC AND HYPOGLYCEMIC CONDITIONS INVITRO
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DOI：
10.1111/j.1471-4159.1988.tb04851.x
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发表时间：
1988-07-01
影响因子：
4.7
通讯作者：
BACHELARD, HS
中科院分区：
文献类型：
--
作者：
GAROFALO, O;COX, DWG;BACHELARD, HS
研究了缺氧和低血糖对体外氧化还原状态的影响。从灌流的大脑皮质切片中同时提取NADH和NAD+，并通过生物发光法进行测定。结果显示，在“轻度缺氧”中，NADH和氧化还原比无显著增加，而“严重缺氧”中，NADH和NADH/NAD+比增加超过200%。当温育培养基中的葡萄糖从其对照值10 mM降低至0.5 mM时，观察到NADH和氧化还原比显著降低至对照值的60%。进一步将葡萄糖降低至0.2 mM，得到较低水平的NADH和氧化还原比（对照的40%）。还测试了对葡萄糖的替代底物的氧化还原状态的影响。用10 mM丙酮酸替代葡萄糖使NADH降低77%，使NADH/NAD+比率降低79%。用10 mM乳酸盐替代葡萄糖分别降低了70%和71%，而在15 mM 2-脱氧葡萄糖和5 mM葡萄糖存在下，NADH降低了56%，比率降低了50%。讨论了与磷酸肌酸和ATP水平以及从平行研究中观察到的诱发动作电位相关的结果。
The effects of hypoxia and hypoglycaemia on the redox state in vitro have been studied. NADH and NAD+, were extracted simultaneously from superfused cerebral cortex slices and assayed by bioluminescence. The results show a nonsignificant increase in NADH and the redox ratio in "mild hypoxia," whereas "severe hypoxia" produced an increase of over 200% in NADH and in the NADH/NAD+ ratio. When the glucose in the incubation medium was reduced from its control value of 10 mM to 0.5 mM, significant decreases in NADH and the redox ratio to 60% of control value were observed. Further decreasing the glucose to 0.2 mM gave lower levels of NADH and the redox ratio (40% of control). The effects on the redox state of alternative substrates to glucose were also tested. Replacement of glucose by 10 mM pyruvate decreased the NADH by 77% and the NADH/NAD+ ratio by 79%. Replacement of glucose with 10 mM lactate gave decreases of 70% and 71%, respectively, whereas in the presence of 15 mM 2-deoxyglucose and 5 mM glucose, the NADH was decreased by 56% and the ratio by 50%. The results are discussed in relation to levels of creatine phosphate and ATP, as well as evoked action potentials, observed from parallel studies.