Specific cleavage of ribosomal RNA and mRNA during victorin-induced apoptotic cell death in oat
Specific cleavage of ribosomal RNA and mRNA during victorin-induced apoptotic cell death in oat
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DOI：
10.1111/j.1365-313x.2006.02752.x
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发表时间：
2006-06-01
期刊：
影响因子：
7.2
通讯作者：
Mayama, Shigeyuki
中科院分区：
文献类型：
--
作者：
Hoat, Trinh X.;Nakayashiki, Hitoshi;Mayama, Shigeyuki
在这里，我们报告rRNA和mRNA的特异性降解燕麦（燕麦L。）在由胜利旋孢菌产生的宿主选择性毒素Victorin诱导的凋亡性细胞死亡过程中，北方分析表明，从细胞质，线粒体和叶绿体的rRNA物种都降解通过特定的降解中间体在维克多林诱导的细胞凋亡，但相反，他们随机消化30 MM硫酸铜和热休克诱导的坏死性细胞死亡。这表明特异性rRNA切割可以由内在程序控制。我们还观察到特定的切割管家基因，如肌动蛋白和泛素在维克多林诱导的细胞死亡的mRNA。有趣的是，在整个实验期间，没有检测到Victorin诱导的mRNA降解与应激反应基因，如PR-1，PR-10和GPx。RNA降解大多数但不总是与DNA梯状化平行发生，但药理学研究表明这些过程受不同信号通路的调节，其中一些上游信号重叠。
Here we report that rRNA and mRNA are specifically degraded in oat (Avena sativa L.) cells during apoptotic cell death induced by victorin, a host-selective toxin produced by Cochliobolus victoriae. Northern analysis indicated that rRNA species from the cytosol, mitochondria and chloroplasts were all degraded via specific degradation intermediates during victorin-induced apoptotic cell death but, in contrast, they were randomly digested in necrotic cell death induced by 30 MM CuSO4 and heat shock. This indicates that specific rRNA cleavage could be controlled by an intrinsic program. We also observed specific cleavage of mRNA of housekeeping genes such as actin and ubiquitin during victorin-induced cell death. Interestingly, no victorin-induced mRNA degradation was detected with stress-responding genes such as PR-1, PR-10 and GPx throughout the experimental period. The RNA degradation mostly, but not always, occurred in parallel with DNA laddering, but pharmacological studies indicated that these processes are regulated by different signaling pathways with some overlapping upstream signals.