Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE ZIM-DOMAIN1 Expression
Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE ZIM-DOMAIN1 Expression
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DOI：
10.1104/pp.114.246694
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发表时间：
2014-12-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Li, Chao;He, Xin;Zhang, Xianlong
植物已经进化出一个复杂的信号网络，以确保适当的免疫反应水平，以满足发育过程的不同需求。先前的研究已经证明DELLA蛋白与JASMONATE ZIM-DOMAIN 1（JAZ 1）物理相互作用，并动态调节赤霉素（GA）和茉莉酸（JA）信号通路的相互作用。然而，JAZ 1-DELLA调节节点在正常生长条件下或在病原体感染期间是否以及如何在植物中在转录水平上被调节尚不清楚。在这里，我们展示了棉花（海岛棉）GbWRKY 1在植物防御反应和发育过程中的多种功能。虽然GbWRKY 1表达被茉莉酸甲酯和大丽轮枝菌感染快速诱导，但我们的研究结果表明，GbWRKY 1是JA介导的防御反应和植物对病原体灰葡萄孢和大丽轮枝菌抗性的负调节因子。在正常生长条件下，GbWRKY 1过表达株系表现出GA相关的表型，包括器官伸长和早花，以及DELLA推定靶点的下调。我们发现，GbWRKY 1过表达植物的GA相关表型依赖于陆地棉GhJAZ 1的组成型表达。我们还表明，GhJAZ 1可以通过TGAC核心序列的GbWRKY 1的反式激活，这个结合位点的相邻序列是必不可少的结合特异性和亲和力GbWRKY 1，所揭示的双荧光素酶报告分析和电泳迁移率变动分析。总之，我们的数据表明，GbWRKY 1是一个关键的调节介导的植物防御发育的过渡在大丽轮枝菌感染期间通过激活JAZ 1的表达。
Plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. Previous research has demonstrated that DELLA proteins physically interact with JASMONATE ZIM-DOMAIN1 (JAZ1) and dynamically regulate the interaction of the gibberellin (GA) and jasmonate (JA) signaling pathways. However, whether and how the JAZ1-DELLA regulatory node is regulated at the transcriptional level in plants under normal growth conditions or during pathogen infection is not known. Here, we demonstrate multiple functions of cotton (Gossypium barbadense) GbWRKY1 in the plant defense response and during development. Although GbWRKY1 expression is induced rapidly by methyl jasmonate and infection by Verticillium dahliae, our results show that GbWRKY1 is a negative regulator of the JA-mediated defense response and plant resistance to the pathogens Botrytis cinerea and V. dahliae. Under normal growth conditions, GbWRKY1-overexpressing lines displayed GA-associated phenotypes, including organ elongation and early flowering, coupled with the down-regulation of the putative targets of DELLA. We show that the GA-related phenotypes of GbWRKY1-overexpressing plants depend on the constitutive expression of Gossypium hirsutum GhJAZ1. We also show that GhJAZ1 can be transactivated by GbWRKY1 through TGAC core sequences, and the adjacent sequences of this binding site are essential for binding specificity and affinity to GbWRKY1, as revealed by dual-luciferase reporter assays and electrophoretic mobility shift assays. In summary, our data suggest that GbWRKY1 is a critical regulator mediating the plant defense-to-development transition during V. dahliae infection by activating JAZ1 expression.