PRODUCTION OF CHIMERAS IN THE DEER MOUSE PEROMYSCUS-MANICULATUS-BAIRDI
PRODUCTION OF CHIMERAS IN THE DEER MOUSE PEROMYSCUS-MANICULATUS-BAIRDI
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DOI：
10.1002/jez.1402180211
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
MARKERT, CL
中科院分区：
文献类型：
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作者：
KLEIN, MS;MARKERT, CL
在鹿鼠（P. maniculatus）中产生种内嵌合体是为了使另一种哺乳动物可用于生殖生物学、发育遗传学和胚胎发育的研究。Peromyscus通常适用于产生Mus musculus嵌合体所采用的相同实验程序和技术。在体外成功地操作和培养了兔胚。受精卵和2-细胞胚胎在体外培养时继续分裂，形成桑椹胚和囊胚。几乎所有聚集的4-和8-细胞胚胎保持在一起并在体外培养1天后发育形成嵌合桑椹胚和囊胚。对M. musculus以适应Peromyscus在生殖生理和行为上的一些差异。必须修改供体雌性和养母的制备方法，以在计划的实验日获得胚胎，并获得假孕养母。有了这些修改后的程序，下午3点。产生Bairdi嵌合体。两个是健康的有生育能力的雄性，皮毛上显示出嵌合体。另一只嵌合体在其养母去世后，在怀孕17天时死亡。通过野生型基因型贡献的黑色色素斑和金发突变基因型贡献的无色素斑的存在，在眼睛的视网膜上皮中检测到嵌合体。开发的程序，使该属的第一嵌合体的生产，使Peromyscus一个合适的动物，在实验室中严格的技术操作。
The production of intraspecific chimeras in the deer mouse, P. maniculatus, was undertaken to make another genus of mammals useful for studies of reproductive biology, developmental genetics and embryonic development. Peromyscus was generally amenable to the same experimental procedures and techniques employed in producing Mus musculus chimeras. Peromyscus embryos could be successfully manipulated and cultured in vitro. Fertilized eggs and 2-cell embryos continue cleavage when cultured in vitro and form morulae and blastocysts. Almost all the 4- and 8-cell embryos that were aggregated remained together and developed, after 1 day of culture in vitro, to form chimeric morulae and blastocysts. Certain changes were made in the chimera-making procedure employed for M. musculus to accommodate some of the differences in reproductive physiology and behavior of Peromyscus. The methods for the preparation of donor females and of foster mothers had to be modified to obtain embryos on the scheduled day of the experiment, and to obtain pseudopregnant foster mothers. With these revised procedures, 3 P. m. bairdi chimeras were produced. Two are healthy fertile males displaying chimerism in the fur. The other chimera was delivered dead at 17 days of gestation, after its foster mother died. Chimerism was detected in the retinal epithelium of the eye by the presence of black pigmented patches contributed by the wild-type genotype, and unpigmented patches, contributed by the blonde mutant genotype. Developing the procedures enabling the production of the 1st chimeras in this genus makes Peromyscus a suitable animal for exacting technical manipulations in the laboratory.