Response surface optimization of culture conditions for improving lutein content in NaCl-stressed germinated corn kernels
Response surface optimization of culture conditions for improving lutein content in NaCl-stressed germinated corn kernels
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提高 NaCl 胁迫发芽玉米粒叶黄素含量培养条件的响应面优化
DOI：
10.1111/jfpp.14130
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发表时间：
2019
影响因子：
2.5
通讯作者：
Xiao Yadong
中科院分区：
文献类型：
--
作者：
Wang Yuxi;He Weiwei;Li Dajing;Baol Yihong;Liu Chunquan;Song Jiangfeng;Xiao Yadong
发芽条件强烈影响玉米籽粒中植物化学成分的含量。在这项研究中，我们优化了NaCl浓度，发芽时间和温度，以最大限度地提高叶黄素的含量。研究了NaCl浓度（X1：150-450 mmol/L）、萌发时间（X2：2-4 d）和萌发温度（X3：20-30°C）对萌发玉米籽粒叶黄素含量（Y）恢复的影响，并采用Box-Behenken设计（BBD）对培养条件进行了优化。结果表明，最佳发芽条件为：NaCl浓度283 mmol/L，发芽时间3.14 d，发芽温度26.5°C。预测叶黄素含量可高达11.77 μg/g DW，比对照高20.95%，是未发芽玉米粒的1.91倍。二次多项式模型显示出较高的决定系数，表明其足以解释实验数据的变化。在优化条件下发芽的玉米可能成为有前途的健康促进食品。实际应用黄色玉米粒含有大量的叶黄素，玉米黄质和其他类胡萝卜素，并已成为越来越受欢迎的零食或蔬菜。发芽过程代表了一种廉价的技术，以提高谷物的营养和保健价值。外源NaCl诱导萌发玉米籽粒中类胡萝卜素含量增加。本研究旨在探讨最佳发芽条件，包括NaCl浓度，发芽时间和温度，以获得最高产量的类胡萝卜素。发芽玉米粒可直接作为功能性蔬菜使用。同时，它还可作为食品加工的原料，提高营养和保健价值，如玉米粉，可用于面条、馒头、饼干等系列产品。
Germination conditions strongly influence the content of phytochemical compounds of corn kernels. In this study, we optimized NaCl concentration, germination time, and temperature for maximizing the content of lutein. The effects of NaCl concentration (X1: 150–450 mmol/L), germination time (X2: 2–4 d), and germination temperature (X3: 20–30°C) on the recovery of lutein content (Y) of germinated corn kernels were investigated and the culture conditions were optimized using a Box–Behenken design (BBD). Results showed that the optimized conditions were 283 mmol/L of NaCl, 3.14 days of germination time, and 26.5°C of germination temperature. The predicted lutein content could be as high as 11.77 μg/g DW, which is 20.95% higher than control and 1.91 times that of non‐germinated corn kernels. The quadratic polynomial model showed high coefficients of determination, demonstrating their adequacy to explain the variations in experimental data. The germinated corn under optimized conditions may become promising health‐promoting foods.Practical applicationsYellow corn kernels contain significant amounts of lutein, zeaxanthin, and other carotenoids and has become increasingly popular as a snack or vegetable. Germination process represents an inexpensive technology to improve nutritional and nutraceutical value of cereal grains. Exogenous NaCl induced the increase in the carotenoid content in the germinated corn kernels. This study was designed to detect optimal germination conditions including NaCl concentration, germination time, and temperature to obtain the highest yields of carotenoids. The germinated corn kernels can be used directly as a functional vegetable. Also, it is used as the raw materials with improved nutritional and nutraceutical value for food processing, such as corn flour, which can be applied in series of products, including noodles, steamed bread, biscuits, etc.