Rapid disruption of intestinal barrier function by gliadin involves altered expression of apical junctional proteins
Rapid disruption of intestinal barrier function by gliadin involves altered expression of apical junctional proteins
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DOI：
10.1016/j.febslet.2005.07.066
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发表时间：
2005-08-29
期刊：
影响因子：
3.5
通讯作者：
Powell, BC
中科院分区：
文献类型：
--
作者：
Sander, GR;Cummins, AG;Powell, BC
乳糜泻是一种慢性肠道疾病，由摄入小麦醇溶蛋白和其他从黑麦和大麦中提取的谷类醇溶蛋白引起。在目前的工作中，我们研究了醇溶蛋白衍生的多肽改变人Caco-2肠上皮细胞屏障功能的机制。我们证明，醇溶蛋白通过降低跨上皮阻力和增加对小分子(4 KDa)的通透性，几乎立即改变屏障功能。醇溶蛋白引起肌动蛋白细丝的重组，并改变紧密连接蛋白occludin、claudin-3和claudin-4、TJ相关蛋白ZO-1和黏附连接蛋白E-cadherin的表达。(C)2005年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Coeliac disease is a chronic enteropathy caused by the ingestion of wheat gliadin and other cereal prolamines derived from rye and barley. In the present work, we investigated the mechanisms underlying altered barrier function properties exerted by gliadin-derived peptides in human Caco-2 intestinal epithelial cells. We demonstrate that gliadin alters barrier function almost immediately by decreasing transepithelial resistance and increasing permeability to small molecules (4 kDa). Gliadin caused a reorganisation of actin filaments and altered expression of the tight junction proteins occludin, claudin-3 and claudin-4, the TJ-associated protein ZO-1 and the adherens junction protein E-cadherin. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.