Diversity of the human intestinal microbial flora
Diversity of the human intestinal microbial flora
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DOI：
10.1126/science.1110591
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发表时间：
2005-06-10
期刊：
影响因子：
56.9
通讯作者：
Relman, DA
中科院分区：
文献类型：
--
作者：
Eckburg, PB;Bik, EM;Relman, DA
人内源性肠菌群是提供滋养，调节上皮的必不可少的“器官”。开发和指导先天免疫；然而，令人惊讶的是，基本特征的描述仍然很差。我们检查了来自多个结肠粘膜位点和健康受试者粪便的13,355个原核生物核糖体RNA基因序列，以提高我们对肠道微生物多样性的理解。大多数细菌序列对应于未栽培的物种和新型微生物。我们发现了粪便和粘膜群落组成之间的显着主体间可变性和差异。这种多样化的生态系统的表征是阐明其在健康和疾病中的作用的第一步。
The human endogenous intestinal microflora is an essential "organ" in providing nourishment, regulating epithelial. development, and instructing innate immunity; yet, surprisingly, basic features remain poorly described. We examined 13,355 prokaryotic ribosomal RNA gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut microbial diversity. A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms. We discovered significant intersubject variability and differences between stool and mucosa community composition. Characterization of this immensely diverse ecosystem is the first step in elucidating its role in health and disease.