Development of hamster tracheal epithelium: II. Cell proliferation in the fetus.
Development of hamster tracheal epithelium: II. Cell proliferation in the fetus.
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仓鼠气管上皮的发育：II。
DOI：
10.1002/ar.1092130310
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Coleman,B
中科院分区：
文献类型：
--
作者：
McDowell,EM;Newkirk,C;Coleman,B
在仓鼠中研究胎儿气管中上皮细胞的增殖，从妊娠第10天开始，到出生后不久的第16天结束。平均有丝分裂指数（MI）在第10天最高，第10天和第11天之间的变化没有统计学证实。MI在第12天和第13天降至约2%，此后在第16天降至约0.3%。比较背侧和腹侧表面的MI，除了第10天腹侧超过背侧和第12天背侧超过腹侧（分别为2.56%和1.3%）外，数值相似。在第10、11和12天，上皮是单层的，由增殖的低分化柱状细胞组成。在第13天，由于存在少数未到达管腔的短细胞，上皮呈假复层。在整个胎儿期，柱状细胞的增殖占主导地位，但分裂短（基底）细胞从8%增加到40%的总有丝分裂活动之间的第13天和第15天。然后基底细胞的增殖下降，因此在第16天，84%的有丝分裂中的所有细胞为柱状。如果基底细胞分裂，使更多的自己，他们必须迅速增殖之间的第13和第15天，因为他们几乎没有在第12天，但约占36%的腹侧和背侧上皮细胞的23%，在第16天。根据这些结果，提出了一个假复层粘膜纤毛上皮形成的假设模型。该模型提出，小颗粒（内分泌）细胞，基底细胞，分泌细胞和纤毛细胞的低分化的柱状细胞的第13天建立。此后，短基细胞复制产生基细胞，而柱状分泌细胞复制产生分泌细胞和纤毛细胞。该模型将在未来的研究中进行测试。
Proliferation of epithelial cells in the fetal trachea was studied in hamsters, beginning on the 10th gestational day and ending on the 16th day, shortly after birth. The mean mitotic index (MI) was highest on day 10, with no statistical confirmation of a change between days 10 and 11. The MI fell to about 2% on days 12 and 13, and declined thereafter to about 0.3% on day 16. The MIs for dorsal and ventral surfaces were compared and values were similar except on day 10, when ventral exceeded dorsal, and on day 12, when dorsal exceeded ventral, 2.56% and 1.3%, respectively. On days 10, 11, and 12 the epithelium was simple, composed of poorly differentiated columnar cells that proliferated. On day 13 the epithelium was pseudostratified owing to the presence of a few short cells that did not reach to the lumen. Throughout the fetal period, proliferation of columnar cells predominated but division of short (basal) cells increased from 8% to 40% of the total mitotic activity between day 13 and day 15. Proliferation of basal cells then declined, so that on day 16, 84% of all cells in mitosis were columnar. If basal cells divide to make more of themselves they must proliferate rapidly between day 13 and day 15, because they were virtually absent on day 12 but accounted for about 36% of the ventral and 23% of the dorsal epithelial cells on day 16. Based on the results,a hypothetical modelis proposed for the formation of pseudostratified mucociliary epithelium. The model proposes that small granule (endocrine) cells, basal cells, secretory cells, and ciliated cells are established from poorly differentiated columnar cells by day 13. Thereafter short basal cells replicate to produceonlybasal cells, whereas columnar secretory cells replicate to produce secretory cellsandciliated cells. This model will be tested in future studies.