Suppression of type IV collagenase in MDA-MB-435 human breast cancer cells by eicosapentaenoic acid in vitro and in vivo.
Suppression of type IV collagenase in MDA-MB-435 human breast cancer cells by eicosapentaenoic acid in vitro and in vivo.
复制标题
二十碳五烯酸在体外和体内抑制 MDA-MB-435 人乳腺癌细胞中的 IV 型胶原酶。
DOI：
10.1016/0304-3835(95)03752-i
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发表时间：
1995
期刊：
影响因子：
9.7
通讯作者：
Rose,DP
中科院分区：
文献类型：
--
作者：
Liu,XH;Rose,DP
饮食中添加4%二十碳五烯酸(EPA)抑制了人乳腺癌细胞MDA-MB-435人乳腺脂肪垫实体瘤在裸鼠体内的肺转移。原发肿瘤匀浆的酶谱分析显示，这种转移抑制与92-kDa IV型胶原酶明胶溶解活性的降低有关；这种异构体以前曾与转移表型相关。72 kDa的异构体也存在，可能来自癌细胞或与肿瘤相关的宿主细胞。在体外，浓度为0.25kDa~1.0kDa g/ml的EPA可浓度依赖性地抑制92-μIV型胶原酶的表达。
Dietary supplementation with 4% eicosapentaenoic acid (EPA), a long-chain polyunsaturated fatty acid, suppressed the development of lung metastases in nude mice from MDA-MB-435 human breast cancer cell mammary fat pad solid tumors. Zymography of primary tumor homogenates showed that this inhibition of metastasis was associated with reduced levels of 92-kDa type IV collagenase gelatinolytic activity; this isoform has been previously associated with the metastatic phenotype. The 72-kDa isoform, which was also present, may have arisen from the cancer cells, or tumor-associated host cells. In vitro, the incorporation of EPA, 0.25–1.0 μg/ml into the medium caused a concentration-related suppression of cultured MDA-MB-435 cell 92-kDa type IV collagenase mRNA expression.