Increased release of fermentable sugars from elephant grass by enzymatic hydrolysis in the presence of surfactants

Increased release of fermentable sugars from elephant grass by enzymatic hydrolysis in the presence of surfactants
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DOI:
10.1016/j.enconman.2014.02.071
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发表时间:
2014-12-01
影响因子:
10.4
通讯作者:
Camassola, Marli
Camassola, Marli
中科院分区:
工程技术1区
文献类型:
--
作者:
Menegol, Daiane;Scholl, Angelica Luisi;Camassola, Marli

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在寻找可再生能源的过程中,象草是生产乙醇的替代底物,但这种底物必须被纤维素酶和木聚糖酶水解以释放可发酵糖。在酶水解过程中,纤维素酶的活性因纤维素酶不可逆吸附在纤维素上而降低,从而降低了水解速率。在水解过程中添加表面活性剂可以改善该过程。在此背景下评估了 Tween (R) 和 Triton (R) 表面活性剂对象草酶水解的影响。数据表明,用氢氧化钠预处理,加上较小的粒径(0.075-0.152 mm)和吐温 80 (R) 的使用,提高了从预处理的象草生物质中释放还原糖的效率。因此,通过使用表面活性剂可以降低第二代乙醇生产中的研磨成本,因为它们可以有效水解较大的生物质颗粒。 (C) 2014 Elsevier Ltd. 保留所有权利。
In the search for renewable energy sources, elephant grass is an alternative substrate for ethanol production, but this substrate must be hydrolyzed by cellulases and xylanases to liberate fermentable sugars. During enzymatic hydrolysis, cellulase activity is reduced by the irreversible adsorption of cellulase onto cellulose, decreasing the rate of hydrolysis. Adding surfactants during hydrolysis can improve the process. The effects of Tween (R) and Triton (R) surfactants on the enzymatic hydrolysis of elephant grass were evaluated in this context. The data indicate that pretreatment with sodium hydroxide, along with a smaller particle size (0.075-0.152 mm) and the use of Tween 80 (R), increased the efficiency of releasing reducing sugars from pretreated elephant grass biomass. Thus, it is possible to reduce grinding costs in second-generation ethanol production through the use of surfactants, as they allow efficient hydrolysis of larger biomass particles. (C) 2014 Elsevier Ltd. All rights reserved.