Induction of VX2 carcinoma in rabbit liver: comparison of two inoculation methods
Induction of VX2 carcinoma in rabbit liver: comparison of two inoculation methods
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DOI：
10.1258/00236770460734434
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发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Han, KW
中科院分区：
文献类型：
--
作者：
Chen, JH;Lin, YC;Han, KW
将VX 2细胞悬液直接注射到肝脏中是简单且广泛使用的。在过去十年中，还开发了使用外科技术将VX 2肿瘤片段植入肝脏的方法。在本研究中，我们比较了这两种方法，以找到建立VX 2肝脏质量的更好方式。40只家兔，体重2.8- 3.2kg，随机分为两组，每组20只。在第1组中，在剖腹手术期间使用27号针头将体积为0.1 ml的含有1 x 10（6）个细胞的肿瘤细胞悬液缓慢注射到肝实质中。在第2组中，将1 mm 3的VX 2癌片段接种到肝左前叶的包膜下。在第1组中，3只家兔显示无肿瘤生长，10只家兔显示肝外渗漏和肿瘤种植的证据。在第2组中，除1只兔外，所有兔均显示肿瘤生长，无肿瘤种植迹象。第1组和第2组的泄漏率分别为50%和0%。总体而言，第1组的接种成功率为35%，第2组为95%。总之，为了在兔中建立VX 2肝肿瘤模型，将VX 2肿瘤片段直接植入肝脏中比将VX 2肿瘤细胞悬液注射到肝脏中获得更好的结果。
Direct injection of VX2 cell suspension into the liver is simple and widely used. Implantation of a fragment of VX2 tumour into the liver using a surgical technique has also been developed in the last decade. In this study, we compared these two methods in order to find a better modality for establishing VX2 liver mass. Forty rabbits, each weighing 2.8-3.2 kg, were divided into two groups, 20 rabbits in each. In Group 1, a tumour cell suspension containing 1 x 10(6) cells in a volume of 0.1 ml, was injected slowly into the liver parenchyma using a 27-gauge needle during laparotomy. In Group 2, a 1 mm 3 fragment of VX2 carcinoma was inoculated into the sub-capsule of the left anterior lobe of the liver. In Group 1, three rabbits showed no tumour growth and 10 rabbits showed evidence of leakage and tumour seeding outside of the liver. In Group 2, all but one rabbit showed tumour growth and none showed evidence of tumour seeding. The leakage rates were 50% and 0% for Group 1 and Group 2, respectively. Overall, the success inoculation rate was 35% for Group 1 and 95% for Group 2. In conclusion, to create the VX2 liver tumour model in rabbits, direct implantation of VX2 tumour fragment into the liver achieved better results than injecting cell suspension of VX2 tumour into the liver.