Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique
Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique
复制标题
基于分级印迹技术制备的蛋白质印迹材料的转铁蛋白识别
DOI：
10.1007/s00604-013-0994-7
复制
发表时间：
2013-11-01
影响因子：
5.7
通讯作者：
Zhang, Yukui
中科院分区：
文献类型：
--
作者：
Li, Qinran;Yang, Kaiguang;Zhang, Yukui
通过分层策略合成了一种新型转铁蛋白印迹聚合物颗粒。首先，通过非共价吸附将转铁蛋白固定在二氧化硅珠上。然后，将由1,4-双（丙烯酰基）哌嗪、甲基丙烯酰胺、甲基丙烯酸、硫酸铵和聚氧乙烯失水山梨糖醇单月桂酸酯组成的预聚合混合物灌注到二氧化硅颗粒的孔中，并在25℃下聚合。最后，用氟化氢铵蚀刻二氧化硅基质，不仅去除模板蛋白，而且暴露出印迹聚合物表面的蛋白质识别位点。转铁蛋白印迹颗粒的结合能力为每克材料6.3毫克蛋白质，达到吸附平衡所需的时间小于10分钟。转铁蛋白的印迹因子约为。 3.3 在核糖核酸酶B、细胞色素c和β-乳球蛋白存在下。结果表明，这些印迹聚合物颗粒可以识别转铁蛋白，具有良好的选择性、高结合能力和快速的传质。它们可以用作人工抗体来去除血浆中的高丰度蛋白质。图通过分层策略合成了一种新型转铁蛋白印迹聚合物颗粒。通过非共价吸附将转铁蛋白固定在二氧化硅珠上。将预聚合混合物灌注到二氧化硅颗粒的孔中并在25℃下聚合。去除二氧化硅基质和模板蛋白后，制备的聚合物表面暴露出蛋白质识别位点，用于转铁蛋白识别
A novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. First, transferrin was immobilized on silica beads by non-covalent absorption. Then, a pre-polymerization mixture, composed of 1,4-bis(acryloyl)piperazine, methacrylamide, methacrylic acid, ammonium sulfate and polyoxyethylene sorbitan monolaurate, was irrigated into the pores of silica particles, and polymerized at 25 °C. Finally, the silica matrix was etched with ammonium hydrogen fluoride, not only to remove the template protein, but also to expose protein recognition sites on the surface of the imprinted polymer. The binding capacity of the transferrin-imprinted particles is 6.3 mg of protein per gram of material, and the time required to reach adsorption equilibrium was less than 10 min. The imprinting factor of transferrin is ca. 3.3 in the presence of ribonuclease B, cytochrome c and β-lactoglobulin. The results indicate that these imprinted polymer particles can recognize transferrin with good selectivity, high binding capacity and fast mass transfer. They may be applied as an artificial antibody to remove the high abundance proteins in plasma.FigureA novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. Transferrin was immobilized on silica beads by non-covalent absorption. The pre-polymerization mixture was irrigated into the pores of silica particles and polymerized at 25°C. After the removal of the silica matrix and template protein, protein recognition sites were exposed on the surface of the prepared polymer and applied for the transferrin recognition