Surface analysis of human plasma fibronectin adsorbed to commercially pure titanium materials.
Surface analysis of human plasma fibronectin adsorbed to commercially pure titanium materials.
复制标题
吸附到商业纯钛材料上的人血浆纤连蛋白的表面分析。
DOI：
10.1002/(sici)1097-4636(199807)41:1
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Boskey,AL
中科院分区：
文献类型：
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作者：
MacDonald,DE;Markovic,B;Allen,M;Somasundaran,P;Boskey,AL
蛋白质结合在金属植入物表面，如钛，是由金属表面的物理化学性质决定的。人血浆纤维连接蛋白(HPF)是一种重要的基质糖蛋白，在创伤愈合过程中介导细胞和蛋白质相互黏附或与细胞外基质黏附。本研究的目的是用原子力显微镜(AFM)和化学分析电子能谱(ESCA)研究HPF在抛光的工业纯钛(CpTi)上的吸附，并测量HPF结合前后的表面接触角。用不同浓度(1μg/m L~10 ng/m L)的HpF溶液与两种不同类型的cpTi盘进行反应，其中一种是在实验室高度抛光的，另一种是市售的(31)。1μg/mLFN涂层样品的ESCA测量光谱显示，与未涂层的对照相比，有机氮和碳的含量增加。原子力显微镜测量HSS和3I cpTi盘的平均接触角分别为36.3°±3.5和39.1°±3.1，表面粗糙度分别为4.45±0.46 nm和22.37±4.17 nm，差异无统计学意义。AFM图像显示，3I试件更加不规则，有大的平行抛光凹槽。吸附的HPF呈球形，平均长16.5±1.0 nm，高2.5±0.5 nm，宽9.6±1.2 nm。纤维连接蛋白涂层在HSS和3I cpTi试件上与未涂层试件相比，疏水性显著增加。这些结果表明了hpf对cpTi的重要意义，并可能解释cpTi种植体的原位功能。1998 John Wiley&Sons，Inc.生物医学杂志，41,120-130,1998。
Protein binding on metallic implant surfaces, such as titanium, is governed by the physico–chemical nature of the metallic surface. Human plasma fibronectin (HPF) is an important matrix glycoprotein that mediates cell and protein attachment to each other or to the extracellular matrix present during wound healing. The objective of this study was to investigate the adsorption of HPF onto polished commercially pure titanium (cpTi) by using atomic force microscopy (AFM) and electron spectroscopy for chemical analysis (ESCA) and to measure the resultant surface contact angle before and after HPF binding. Two types of cpTi disks, one highly polished in our laboratory (HSS) and one commercially prepared (31), were reacted with HPF solutions of varying concentrations (1 μg/mL—10 ng/mL). ESCA survey spectra of samples coated with 1 μg/mL of fibronectin showed an increase in organic nitrogen and carbon compared with uncoated controls. Contact angle measurements of HSS and 3I cpTi disks showed no significant difference in average contact angle (36.3° ± 3.5 and 39.1° ± 3.1) despite differences in local root mean square (RMS) surface roughness (4.45 ± 0.46 nm and 22.37 ± 4.17 nm) as measured by AFM. Images obtained by AFM showed that 3I specimens were more irregular, with large parallel polishing grooves. Adsorbed HPF appeared in a globular form with an average length of 16.5 ± 1.0 nm, a height of 2.5 ± 0.5 nm, and a width of 9.6 ± 1.2 nm. Fibronectin coating on both HSS and 3I cpTi specimens resulted in a significant increase in hydrophobicity compared to uncoated specimens. These results indicate the significance of HPF on cpTi and may explain how cpTi implants functionin situ. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 41, 120–130, 1998.