Multiple gene sequences delimit Botryosphaeria australis sp. nov. from B. lutea
Multiple gene sequences delimit Botryosphaeria australis sp. nov. from B. lutea
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DOI：
10.1080/15572536.2005.11832903
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发表时间：
2004-09
期刊：
影响因子：
2.8
通讯作者：
B. Slippers;G. Fourie;P. Crous;T. Coutinho;B. Wingfield;M. Wingfield
中科院分区：
文献类型：
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作者：
B. Slippers;G. Fourie;P. Crous;T. Coutinho;B. Wingfield;M. Wingfield
黄色葡萄座腔菌（无性型黄色梭孢菌）最容易与其他葡萄座腔菌属区分开来。在年轻的文化中形成的黄色色素。据报道，这种真菌来自新西兰和葡萄牙的一些栽培宿主。在对发生在澳大利亚本地金合欢属物种上的葡萄座腔菌属真菌的调查中，在一些培养物中观察到黄色色素。这些分离株在形态上与B相似。但色素与正品B形成的色素略有不同。分离的黄曲霉初步数据还显示ITS rDNA序列数据的微小差异。本研究的目的是确定这些微小的差异是否表明不同的物种或仅仅是B内的变异。lutea比较了B的无性型、有性型和培养形态。lutea和Acacia分离物来自澳大利亚。将β-tubulin和EF 1-α基因及内含子区的序列数据与ITS rDNA序列数据相结合，以确定这些菌株之间的系统发育关系。B的分离株。lutea和来自澳大利亚金合欢属物种的那些在孢子形态上没有显著差异。然而，在B的培养物中，黄色色素要明显得多。lutea比从相思文化。在每个分析的基因区域中只有少数碱基对变异，但这些变异在所有区域的两组中是固定的。通过结合这些数据，很明显，B。lutea和来自金合欢属的分离物是不同的物种，尽管非常密切相关。因此，我们提出了新的修饰语B。australis为澳大利亚的真菌。在这项研究中，还从澳大利亚的外来红杉中分离到了南方葡萄座腔菌。对GenBank数据的重新分析表明，B. australis也发生在其他澳大利亚本土宿主上，即Banksia sp.和Eucalyptus sp.，以及南非的原生Protea sp.和意大利的开心果上。这些来自GenBank的记录先前已被鉴定为B。lutea常见的B。australis在澳大利亚各地的各种本地宿主上的生长表明这种真菌原产于该地区。
Botryosphaeria lutea (anamorph Fusicoccum luteum) most easily is distinguished from other Botryosphaeria spp. by a yellow pigment that is formed in young cultures. This fungus has been reported from a number of cultivated hosts in New Zealand and Portugal. During a survey of Botryosphaeria fungi that occur on native Acacia species in Australia, a yellow pigment was observed in some cultures. These isolates were morphologically similar to B. lutea, but the pigment differed slightly from the one formed by authentic B. lutea isolates. Preliminary data also revealed small differences in ITS rDNA sequence data. The aim of this study was to determine whether these small differences were indicative of separate species or merely variations within B. lutea. Anamorph, teleomorph and culture morphology were compared between B. lutea and Acacia isolates from Australia. Sequence data of two other genome regions, namely the β-tubulin and EF1-α gene and intron regions, were combined with ITS rDNA sequence data to determine the phylogenetic relationship between these isolates. Isolates of B. lutea and those from Australian Acacia species were not significantly different in spore morphology. The yellow pigment, however, was much more distinct in cultures of B. lutea than in cultures from Acacia. There were only a few base pair variations in each of the analyzed gene regions, but these variations were fixed in the two groups in all regions. By combining these data it was clear that B. lutea and the isolates from Acacia were distinct species, albeit very closely related. We, therefore, propose the new epithet B. australis for the fungus from Australia. Botryosphaeria australis also was isolated in this study from exotic Sequoiadendron trees in Australia. Re-analyses of GenBank data in this study showed that B. australis also occurs on other native Australian hosts, namely a Banksia sp. and a Eucalyptus sp., as well as a native Protea sp. in South Africa and on Pistachio in Italy. These records from GenBank have been identified previously as B. lutea. The common occurrence of B. australis on a variety of native hosts across Australia suggests that this fungus is native to this area.