Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cells.

Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cells.
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佛波酯处理的 3T3 细胞中 Ca2 敏感性、磷脂依赖性蛋白激酶活性消失。

DOI:
10.1016/s0006-291x(84)80213-2
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发表时间:
1984
影响因子:
3.1
通讯作者:
E. Rozengurt
E. Rozengurt
中科院分区:
生物学4区
文献类型:
--
作者:
A. Rodríguez;E. Rozengurt

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用生物活性佛波醇酯处理完整的3 T3细胞会导致在无细胞、洗涤剂溶解的提取物中测得的Ca 2+敏感性、磷脂依赖性蛋白激酶(蛋白激酶C)的总活性逐渐下降。该效应是剂量依赖性和可逆的;当从培养基中除去佛波酯时,蛋白激酶C活性重新出现。
Treatment of intact 3T3 cells with biologically active phorbol esters causes a progressive decline in the total activity of Ca2+-sensitive, phospholipid-dependent protein kinase (protein kinase C) measured in cell-free, detergent-solubilized, extracts. The effect is dose-dependent and reversible; protein kinase C activity reappears when the phorbol esters are removed from the medium.
DOI: 10.1016/s0065-230x(08)60360-7
发表时间: 1980-01-01
影响因子: --
作者:
Diamond, L;O'Brien, T G;Baird, W M
通讯作者: Baird, W M
DOI: --
发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
作者:
Phillips,MA;Jaken,S
通讯作者: Jaken,S