Oxygenation of the cerebrospinal fluid with artificial cerebrospinal fluid can ameliorate a spinal cord ischemic injury in a rabbit model
Oxygenation of the cerebrospinal fluid with artificial cerebrospinal fluid can ameliorate a spinal cord ischemic injury in a rabbit model
复制标题
DOI：
10.1016/j.jtcvs.2016.04.095
复制
发表时间：
2016-11-01
影响因子：
6
通讯作者：
Saiki, Yoshikatsu
中科院分区：
文献类型：
--
作者：
Kanda, Keisuke;Adachi, Osamu;Saiki, Yoshikatsu
目的：观察脑脊液氧合对兔肾下主动脉阻塞后脊髓缺血性损伤的预防作用。方法：将20只大白兔随机分为4组，每组5只：S组（假手术），球囊导管置入主动脉；C组（对照组），肾下腹主动脉球囊阻断脊髓缺血性损伤15分钟；N组（无氧），脊髓缺血性损伤，用无氧人工脑脊液替代脑脊液；O组（氧合组），脊髓缺血性损伤，用纳米气泡含氧人工脑脊液替代脑脊液。观察缺血围期脑脊液氧分压变化、改良Tarlov评分及主动脉操作后48小时脊髓组织病理学变化。结果：脑脊液置换后，O组脑脊液氧分压明显高于N组（254.5 +/- 54.8 mm Hg vs 136.1 +/- 43.5 mm Hg, P = 0.02）。脊髓缺血性损伤15分钟后，C组脑脊液氧分压较基线（148.8 +/- 20.6 mm Hg, P < 0.01）降至65.8 +/- 18.6 mm Hg，而O组脑脊液氧分压在脊髓缺血性损伤后维持在较高水平（291.9 +/- 51.8 mm Hg），与神经功能改善有关。经组织病理学分析证实，N组和O组保存了前角神经元，退行性神经元较c组明显减少。结论：人工脑脊液充氧对家兔脊髓缺血性损伤有保护作用。
Objective: We evaluated the effect of cerebrospinal fluid oxygenation for the prevention of spinal cord ischemic injury after infrarenal aortic occlusion in a rabbit model.Methods: Twenty white Japanese rabbits were categorized into the following 4 groups (5 in each): group S (sham), balloon catheter insertion on to the aorta; group C (control), spinal cord ischemic injury by infrarenal abdominal aortic balloon occlusion for 15 minutes; group N (nonoxygenated), spinal cord ischemic injury with cerebrospinal fluid replacement by nonoxygenated artificial cerebrospinal fluid; and group O (oxygenated), spinal cord ischemic injury with cerebrospinal fluid replacement by nanobubble-oxygenated artificial cerebrospinal fluid. The changes in cerebrospinal fluid partial pressure of oxygen during the periischemic period, modified Tarlov score, and histopathology of the spinal cord 48 hours after aortic maneuvers were evaluated.Results: Cerebrospinal fluid partial pressure of oxygen significantly increased in group O compared with group N after cerebrospinal fluid replacement (254.5 +/- 54.8 mm Hg vs 136.1 +/- 43.5 mm Hg, P = .02). After 15 minutes of spinal cord ischemic injury, cerebrospinal fluid partial pressure of oxygen in group C decreased to 65.8 +/- 18.6 mm Hg compared with baseline (148.8 +/- 20.6 mm Hg, P < .01), whereas cerebrospinal fluid partial pressure of oxygen in group O was maintained at remarkably high levels after spinal cord ischemic injury (291.9 +/- 51.8 mm Hg), which was associated with improved neurologic function, with 20% of spinal cord ischemic injury having a Tarlov score less than 5 compared with 100% of spinal cord ischemic injury in group C. Preservation of anterior horn neurons in groups N and O was confirmed by histopathologic analysis with significant reduction of degenerated neurons compared with group C.Conclusions: Cerebrospinal fluid oxygenation with artificial cerebrospinal fluid can exert a protective effect against spinal cord ischemic injury in rabbits.