Genetic analysis and gene fine mapping for a rice novel mutant (rl9(t)) with rolling leaf character
Genetic analysis and gene fine mapping for a rice novel mutant (rl9(t)) with rolling leaf character
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DOI：
10.1007/s11434-005-1142-5
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发表时间：
2006
影响因子：
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通讯作者：
Changjie Yan;Song Yan;Zhengqiu Zhang;G. Liang;Jufei Lu;M. Gu
中科院分区：
文献类型：
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作者：
Changjie Yan;Song Yan;Zhengqiu Zhang;G. Liang;Jufei Lu;M. Gu
叶形是水稻表型育种的重要参数，叶形的卷曲也有利于籽粒的高效成熟。这鼓励了对调节叶子形状的新基因的探索。本研究对粳稻品种中华11中鉴定出的新型卷叶突变体进行了遗传分析和基因定位。SSR标记分析表明该突变体由位于9号染色体上的单个隐性基因(rl9(t))控制。基于日本晴和93-11之间的序列多样性，利用围绕Rl9(t)锚定区开发的30个新的STS标记对Rl9(t)位点进行了精细定位。精细作图需要构建包含R19(t)基因座的PAC重叠群，其被界定为42kb区域。因此，这可以在进一步的研究中增强目标基因的克隆。
Leaf shape is an important parameter for ideotype breeding in rice, and the rolling of leaf is also beneficial to efficient ripening of grains. This encourages the explorations of new genes that regulate leaf shape. In this study, genetic analysis and gene mapping were carried out for a novel rolling leaf mutant identified fromjaponicavariety Zhonghua 11. The SSR marker analysis showed that the mutant was controlled by a single recessive gene (rl9(t)) located on chromosome 9. Fine mapping of theRl9(t)locus was conducted with 30 new STS markers developed aroundRl9(t)anchored region based on the sequence diversity between Nipponbare and 93–11. The fine mapping necessitated the contruction of a PAC contig encompassing theRl9(t)locus, which was delimited to a 42 kb region. This could therefore enhance the cloning of the target gene in further studies.