SCARECROW-LIKE15 interacts with HISTONE DEACETYLASE19 and is essential for repressing the seed maturation programme.
SCARECROW-LIKE15 interacts with HISTONE DEACETYLASE19 and is essential for repressing the seed maturation programme.
复制标题
类似稻草人的15与组蛋白脱乙酰基酶相互作用，对于抑制种子成熟程序至关重要。
DOI：
10.1038/ncomms8243
复制
发表时间：
2015-07-01
影响因子：
16.6
通讯作者：
Hegedus DD
中科院分区：
文献类型：
--
作者：
Gao MJ;Li X;Huang J;Gropp GM;Gjetvaj B;Lindsay DL;Wei S;Coutu C;Chen Z;Wan XC;Hannoufa A;Lydiate DJ;Gruber MY;Chen ZJ;Hegedus DD
基因表达的表观遗传调控是控制胚胎到幼苗阶段的胚胎特性的关键。在这里，我们报道了一种与HISTONE DEACETYLASE19 （HDA19）相关的调节因子，稻草人- like15 (SCL15)，在抑制营养组织中的种子成熟程序中是必不可少的。sc15表达于维管束中，gfp标记的sc15主要定位于维管束，特别是韧皮部伴细胞和邻近的特化细胞。SCL15的突变导致幼苗中基因表达的全局转变，类似于种子胚胎发生晚期。在scl15幼苗中，许多与种子成熟有关的基因被显著抑制，并伴随12S球蛋白的积累；这与种子特异性位点的组蛋白乙酰化水平升高有关。SCL15与HDA19物理相互作用，并确定了HDA19 - SCL15关联的直接靶点。这些研究表明，SCL15作为hda19相关的调节因子抑制幼苗的胚胎性状。表观遗传调控是控制植物从胚胎到幼苗过渡过程中基因表达的关键。在这里，Gao等人报道，在拟南芥中，稻草人- like15蛋白与组蛋白去乙酰化酶相互作用，抑制幼苗中胚胎性状的表达。
Epigenetic regulation of gene expression is critical for controlling embryonic properties during the embryo-to-seedling phase transition. Here we report that a HISTONE DEACETYLASE19 (HDA19)-associated regulator, SCARECROW-LIKE15 (SCL15), is essential for repressing the seed maturation programme in vegetative tissues. SCL15 is expressed in and GFP-tagged SCL15 predominantly localizes to, the vascular bundles particularly in the phloem companion cells and neighbouring specialized cells. Mutation of SCL15 leads to a global shift in gene expression in seedlings to a profile resembling late embryogenesis in seeds. In scl15 seedlings, many genes involved in seed maturation are markedly derepressed with concomitant accumulation of seed 12S globulin; this is correlated with elevated levels of histone acetylation at a subset of seed-specific loci. SCL15 physically interacts with HDA19 and direct targets of HDA19–SCL15 association are identified. These studies reveal that SCL15 acts as an HDA19-associated regulator to repress embryonic traits in seedlings. Epigenetic regulation is critical for controlling gene expression during the transition from embryo to seedling in plants. Here Gao et al. report that in Arabidopsis, the SCARECROW-LIKE15 protein physically interacts with a histone deacetylase to repress expression of embryonic traits in seedlings.