Responsive behavior of tumor-marker-imprinted hydrogels using macromolecular cross-linkers
Responsive behavior of tumor-marker-imprinted hydrogels using macromolecular cross-linkers
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DOI：
10.1002/jmr.2190
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发表时间：
2012-06-01
影响因子：
2.7
通讯作者：
Uragami, Tadashi
中科院分区：
文献类型：
--
作者：
Miyata, Takashi;Hayashi, Takeshi;Uragami, Tadashi
具有凝集素和抗体分子作为肿瘤特异性标志物糖蛋白的配体的肿瘤标志物印迹水凝胶通过使用微量的低分子量或高分子量交联剂的生物分子印迹策略性地制备。肿瘤标记物印迹水凝胶响应于靶糖蛋白而逐渐收缩，因为它们的表观交联密度增加，这是由于凝集素和抗体配体在它们的配体动态识别糖蛋白后与靶糖蛋白同时形成复合物。使用具有最佳链长的高分子量交联剂的肿瘤标记物印迹水凝胶的溶胀比比使用低分子量交联剂的溶胀比更急剧地降低，但是使用具有太长链的交联剂的水凝胶没有表现出肿瘤标记物响应行为。本文主要研究了交联剂分子量对以凝集素和抗体分子为配体的肿瘤标志物印迹水凝胶响应行为的影响。交联剂链长是影响生物分子印迹水凝胶对糖蛋白动态识别和响应行为的重要因素。版权所有（c）2012约翰威利父子有限公司
Tumor-marker-imprinted hydrogels having lectin and antibody molecules as ligands for a tumor-specific marker glycoprotein were strategically prepared by biomolecular imprinting using minute amounts of low-molecular-weight or high-molecular-weight cross-linkers. The tumor-marker-imprinted hydrogels shrank gradually in response to a target glycoprotein, because their apparent cross-linking density increased owing to simultaneous complex formation of lectin and antibody ligands with a target glycoprotein after their ligands dynamically recognized the glycoprotein. The swelling ratio of the tumor-marker-imprinted hydrogel using high-molecular-weight cross-linker with an optimal chain length decreased more drastically than that using a low-molecular-weight cross-linker, but the hydrogel using the cross-linker with a chain that was too long did not exhibit tumor-marker responsive behavior. This paper focuses on the effect of the molecular weight of cross-linkers on the responsive behavior of tumor-marker-imprinted hydrogels having lectin and antibody molecules as ligands. The cross-linker chain length was an important factor in determining the dynamic glycoprotein recognition and responsive behavior of the biomolecule-imprinted hydrogels. Copyright (c) 2012 John Wiley & Sons, Ltd.