Vesicle formation from the nuclear membrane is induced by coexpression of two conserved herpesvirus proteins
Vesicle formation from the nuclear membrane is induced by coexpression of two conserved herpesvirus proteins
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DOI：
10.1073/pnas.0701757104
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发表时间：
2007-04-24
影响因子：
11.1
通讯作者：
Mettenleiter, Thomas C.
中科院分区：
文献类型：
--
作者：
Klupp, Barbara G.;Granzow, Harald;Mettenleiter, Thomas C.
尽管核膜是一种在有丝分裂过程中分解和重组的动态结构，但在未感染的细胞中尚未描述源自核膜的膜囊泡的形成。然而，在疱疹病毒成熟过程中，核内衣壳通过在内核膜处出芽开始转运至胞质溶胶以最终成熟。该初级包膜需要两种保守的疱疹病毒蛋白，在α疱疹病毒中称为pUL31和pUL34。在这里，我们发现在稳定转染的兔肾细胞中同时表达α疱疹病毒伪狂犬病病毒的pUL31和pUL34导致核周空间中形成囊泡，类似于没有核衣壳的初级被膜。免疫标记显示它们含有 pUL31 和 pUL34，并且源自核膜。因此，仅两种保守的疱疹病毒蛋白在没有任何其他病毒因子的情况下共表达就足以诱导从核膜形成囊泡。这证明了细胞因子在此过程中的贡献，这些细胞因子要么是从其天然细胞质位置招募的，要么尚未被鉴定为核区室的组成部分。
Although the nuclear envelope is a dynamic structure that disassembles and reforms during mitosis, the formation of membranous vesicles derived from the nuclear envelope has not yet been described in noninfected cells. However, during herpesvirus maturation, intranuclear capsids initiate transit to the cytosol for final maturation by budding at the inner nuclear membrane. Two conserved herpesvirus proteins are required for this primary envelopment, designated in the alphaherpesviruses as pUL31 and pUL34. Here, we show that simultaneous expression of pUL31 and pUL34 of the alphaherpesvirus pseudorabies virus in stably transfected rabbit kidney cells resulted in the formation of vesicles in the perinuclear space that resemble primary envelopes without a nucleocapsid. They contain pUL31 and pUL34 as shown by immunolabeling and are derived from the nuclear envelope. Thus, coexpression of only two conserved herpesvirus proteins without any other viral factor is sufficient to induce the formation of vesicles from the nuclear membrane. This argues for the contribution of cellular factors in this process either recruited from their natural cytoplasmic location or not yet identified as components of the nuclear compartment.