NEUTRALIZING EPITOPES OF TYPE-O FOOT-AND-MOUTH-DISEASE VIRUS .1. IDENTIFICATION AND CHARACTERIZATION OF 3 FUNCTIONALLY INDEPENDENT, CONFORMATIONAL SITES
NEUTRALIZING EPITOPES OF TYPE-O FOOT-AND-MOUTH-DISEASE VIRUS .1. IDENTIFICATION AND CHARACTERIZATION OF 3 FUNCTIONALLY INDEPENDENT, CONFORMATIONAL SITES
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DOI：
10.1099/0022-1317-70-6-1483
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发表时间：
1989-06-01
影响因子：
3.8
通讯作者：
PARRY, NR
中科院分区：
文献类型：
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作者：
BARNETT, PV;OULDRIDGE, EJ;PARRY, NR
11中和单克隆抗体（MAbs）的生产O 1BFS 1860/67株的口蹄疫病毒（FMDV），其特征在于它们的能力，结合病毒和亚病毒抗原在不同的ELISA试验和中和异源O型分离物。在压力下从这些单克隆抗体中分离的同源病毒的中和逃逸变体用于与所有11种抗体的交叉中和试验。这些研究确定了三个功能独立的，构象，中和位点。构象依赖性最强的位点结合抗体可中和一系列O型病毒分离株。第二个位点对构象的依赖性较小，并被菌株特异性抗体识别。最少构象位点结合的单克隆抗体显示出有限的交叉中和其他O型菌株。后一个位点似乎是免疫显性的，包含几个重叠的表位，这些表位在其特异性上显示出一些差异。变体的等电聚焦和测序研究强烈表明多肽VP 2对免疫显性位点有贡献。
Eleven neutralizing monoclonal antibodies (MAbs) were produced to the O1BFS 1860/67 strain of foot-and-mouth disease virus (FMDV), and were characterized for their ability to bind viral and subviral antigens in different ELISA tests and to neutralize heterologous type O isolates. Neutralization escape variants of the homologous virus, isolated under pressure from the of these MAbs, were used in cross-neutralization tests with all of the 11 antibodies. These studies identified three functionally independent, conformational, neutralizing sites. The most conformationally dependent site bound antibody which neutralized a range of type O virus isolates. A second site was less dependent on conformation and was recognized by antibody that was strain-specific. The least conformational site bound MAbs which showed limited cross-neutralization of other type O strains. This latter site appeared to be immunodominant and contained several overlapping epitopes which showed some differences in their specificities. Isoelectrofocusing and sequencing studies of the variants strongly suggested that polypeptide VP2 contributes to the immunodominant site.