Isolation and characterization of bovine brain myelin distribution of 5′-nucleotidase
Isolation and characterization of bovine brain myelin distribution of 5′-nucleotidase
复制标题
5′-核苷酸酶牛脑髓磷脂分布的分离和表征
作者：
V. Casadó;J. Mallol;J. Bozal
通过几种公开发表的程序和对这些程序的修改，从牛脑中分离出髓鞘。脑白质匀浆中髓鞘标记物(2‘，3’-环核苷酸3‘-磷酸水解酶)活性高，污染物标记物活性低，说明白质分离髓鞘优于大脑皮层或全脑。本文介绍了一种从牛白质中制备精制髓鞘的方法，其精制髓鞘中的蛋白质含量(40%)、髓鞘标志物(51%)和5‘-核苷酸酶(25%)的含量比任何方法都高。脑白质匀浆中乙酰胆碱酯酶或琥珀酸脱氢酶活性低于7%，髓鞘部分未恢复单胺氧化酶和NADPH：细胞色素c还原酶。在形态上，髓鞘组分主要由大小不一的多层膜组成。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法显示髓鞘组分呈典型的髓鞘蛋白图谱。当我们的程序应用于冷冻白质时，髓鞘中较低的蛋白质(32%)和髓鞘标志物(34%)以及类似的5‘-核苷酸酶活性(24%)被恢复，在白质密度较高的部分增加了其恢复。
Myelin was isolated from bovine brain by several published procedures and modifications of these procedures. High activity of the myelin marker (2′,3′-cyclic nucleotide 3′-phosphohydrolase) and low activity of contaminants markers in white matter homogenates in respect to cerebral cortex showed the white matter to be better than the cerebral cortex or the whole brain for myelin isolation. A procedure is described for the preparation of purified myelin from bovine white matter which yielded a content of protein (40%), myelin marker (51%), and 5′-nucleotidase (25%) in purified myelin higher than by any used method. Acetylcholinesterase or succinate dehydrogenase was lower than 7% of its activity in the white matter homogenate, and monoamine oxidase and NADPH: cytochrome c reductase were not recovered in myelin fraction. Morphologically, myelin fraction was shown to mainly consist of multilamellar membranes of different sizes. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of myelin fraction showed a characteristic protein pattern of myelin. When our procedure was applied to frozen white matter, lower protein (32%) and myelin marker (34%) and similar 5′-nucleotidase activity (24%) were recovered in myelin, increasing its recovery in denser fractions of white matter.
大鼠脑 5-核苷酸酶：髓磷脂的发育变化以及亚细胞组分和髓磷脂亚组分的活性。
影响因子：
2.9
作者：
Cammer,W;ZimmermanJr,TR
通讯作者：
ZimmermanJr,TR