Use of 75Se-labeled methionine to study the sequestration of senescent red blood cells.
Use of 75Se-labeled methionine to study the sequestration of senescent red blood cells.
复制标题
使用 75Se 标记的蛋氨酸研究衰老红细胞的隔离。
DOI：
10.1002/ajh.2830180106
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发表时间：
1985
影响因子：
12.8
通讯作者：
Aminoff,D
中科院分区：
文献类型：
--
作者：
Smedsrød,B;Aminoff,D
用 Na251CrO4 标记红细胞使我们能够研究红细胞存活和循环隔离的某些方面。然而，作为随机标记程序，51Cr 方法具有一定的局限性。因此，我们开发了一种使用 75Se-蛋氨酸作为双鼠程序的队列标记方法。这为我们提供了清晰的脉冲标记的大鼠红细胞群，以研究隔离的动态。通过这种标记程序，可以证明：1) 红细胞密度随着年龄的增长而增加，2) 红细胞从循环中的显着隔离在 48 天结束时很明显，并在 60 天结束时基本完成，3) 脾脏中有相应的衰老红细胞吸收，在 55 天时达到峰值，4) 60 天终点更尖锐，更明确，当将脾脏中标记的红细胞的“比活性”（每个红细胞的cpm）与仍在循环中的红细胞的“比活性”进行比较。 Asialo 红细胞是通过唾液酸酶去除唾液酸获得的，经常被用作研究衰老红细胞隔离的模型。利用本文描述的技术，可以证明虽然去唾液酸红细胞会抑制标记的去唾液酸红细胞的摄取，但它们对衰老红细胞的隔离没有影响。据推测，涉及不同的封存位点和机制。
Labeling red blood cells with Na251CrO4enabled us to study certain aspects of red cell survival and sequestration from the circulation. As a random labeling procedure, however, the51Cr method has certain limitations. Therefore, we developed a cohort labeling method using75Se‐methionine as a two‐rat procedure. This gives us a clear pulse‐labeled population of rat red cells to study the dynamics of sequestration. With this labeling procedure, it was possible to demonstrate that 1) there is an increase in the density of red cells with age, 2) a significant sequestration of red cells from the circulation is apparent at the end of 48 days and essentially is complete at the end of 60 days, 3) there is a corresponding uptake of senescent red cells in the spleen, which peaks at 55 days, and 4) the 60‐day end point is sharper and is more definitive when the “specific activity” (cpm per red blood cell) of the labeled red cells in the spleen is compared to that of the red cells still in the circulation. Asialo red cells, obtained by removal of sialic acid with sialidase, frequently have been used as a model for the study of sequestration of senescent red cells. With the technique herein described, it was possible to show that while asialo red cells will inhibit the uptake of labeled asialo red cells, they have no effect on the sequestration of senescent red cells. Presumably, different sites and mechanisms of sequestration are involved.