Serum amyloid A does not affect high-density lipoprotein cholesterol measurement by a homogeneous assay.
Serum amyloid A does not affect high-density lipoprotein cholesterol measurement by a homogeneous assay.
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DOI：
10.1016/j.clinbiochem.2018.10.008
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发表时间：
2019-01
影响因子：
2.8
通讯作者：
Megumi Sato;R. Ohkawa;Hann Low;Madoka Nishimori;S. Okubo;Akira Yoshimoto;K. Yano;T. Kameda;Y. Yatomi;M. Tozuka
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文献类型：
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作者：
Megumi Sato;R. Ohkawa;Hann Low;Madoka Nishimori;S. Okubo;Akira Yoshimoto;K. Yano;T. Kameda;Y. Yatomi;M. Tozuka
血清淀粉样蛋白A （SAA）是急性期蛋白之一，在循环过程中通过与HDL结合改变其结构。我们关注的是SAA在使用均相分析时是否会影响高密度脂蛋白胆固醇（HDL-C）的测量值。MethodsHDLs超速离心法分离的血清样本的248例患者分为三组根据他们的血清SAA含量(低:SAA ≤ 8 μg / mL;中间:8 < SAA ≤100 μg / mL;和高:SAA > 100年 μg / mL)。将均相法测定的血清样品的HDL- c浓度与超离心分离的HDL馏分的总胆固醇浓度进行比较。结果从低SAA浓度的患者中获得的shdl通过天然凝胶电泳分离到一般粒径，并分为hdl2和hdl3。另一方面，高SAA浓度患者的hdl偶尔表现出与hdl2和HDL3典型大小不同的分布，如极小或极大的颗粒。然而，无论SAA浓度如何，均相法测定的HDL-C浓度与超离心法测定的HDL-C浓度密切相关。然而，SAA浓度高的患者，均相法测定的HDL-C浓度与超离心法测定的HDL-C浓度之比明显低于SAA浓度低的患者。结论大量SAA附着在HDL上可改变HDL的粒径，但对均相法测定HDL- c无明显影响。
BackgroundSerum amyloid A (SAA), which is one of the acute phase proteins, alters the structure of HDL by associating with it during circulation. We focused on whether SAA influences the values of HDL-cholesterol (HDL-C) measurements when using a homogeneous assay.MethodsHDLs were isolated by ultracentrifugation from serum samples of 248 patients that were stratified into three groups based on their serum SAA concentrations (low: SAA ≤ 8 μg/mL; middle: 8 < SAA ≤ 100 μg/mL; and high: SAA > 100 μg/mL). HDL-C concentrations of the serum samples measured by the homogeneous assay were compared with the total cholesterol concentrations of HDL fractions isolated by ultracentrifugation.ResultsHDLs obtained from patients with low SAA concentrations were separated into their general particle sizes and classified as HDL2and HDL3by native-gel electrophoresis. On the other hand, HDLs obtained from patients with high SAA concentrations occasionally showed distributions different from the typical sizes of HDL2and HDL3, such as extremely small or large particles. Nevertheless, HDL-C concentrations measured using the homogeneous assay were strongly correlated with those measured using the ultracentrifugation method, regardless of the SAA concentrations. However, the ratios of HDL-C concentrations obtained by the homogeneous assay to those obtained by the ultracentrifugation method for patients with high SAA concentrations were significantly lower than those of patients with low SAA concentrations.ConclusionsA large amount of SAA attached to HDL altered the HDL particle size but did not essentially affect HDL-C measurement by homogeneous assay.