Collagen in the scarless fetal skin wound: Detection with Picrosirius-polarization
Collagen in the scarless fetal skin wound: Detection with Picrosirius-polarization
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DOI：
10.1111/j.1067-1927.2005.130211.x
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发表时间：
2005-03-01
影响因子：
2.9
通讯作者：
Hayes, MT
中科院分区：
文献类型：
--
作者：
Cuttle, L;Nataatmadja, M;Hayes, MT
我们的小组已经开发了一个绵羊模型的深真皮，部分厚度烧伤，其中胎儿愈合无疤痕和羔羊愈合疤痕。比较两种不同愈合机制的胶原结构，可以阐明无瘢痕愈合的过程。使用天狼星苦染色，然后使用偏振光显微镜观察胶原纤维，并进行数字捕获和分析。胶原沉积随着胎龄增加，纤维变粗，从绿色（III型胶原）变为黄色/红色（I型胶原）。胎儿中III型胶原与I型胶原的比例很高（166），而羔羊的比例要低得多（0.2）。烧伤后，III型胶原蛋白与I型胶原蛋白的比例与胎儿或羔羊的对照皮肤没有差异。烧伤后胎羊组织保持正常的组织结构，而羔羊组织显示不规则的胶原组织。总之，胶原蛋白的类型或数量在损伤后没有显著改变。胎儿和羔羊组织的组织结构不同，表明瘢痕的发展与胶原交联或排列有关。这项研究表明，在无瘢痕胎儿伤口愈合是正常的胎儿生长模式的代表，而不是一个“响应”烧伤。
Our group has developed an ovine model of deep dermal, partial-thickness burn where the fetus heals scarlessly and the lamb heals with scar. The comparison of collagen structure between these two different mechanisms of healing may elucidate the process of scarless wound healing. Picrosirius staining followed by polarized light microscopy was used to visualize collagen fibers, with digital capture and analysis. Collagen deposition increased with fetal age and the fibers became thicker, changing from green (type III collagen) to yellow/red (type I collagen). The ratio of type III collagen to type I was high in the fetus (166), whereas the lamb had a much lower ratio (0.2). After burn, the ratios of type III to type I collagen did not differ from those in control skin for either fetus or lamb. The fetal tissue maintained normal tissue architecture after burn while the lamb tissue showed irregular collagen organization. In conclusion, the type or amount of collagen does not alter significantly after injury. Tissue architecture differed between fetal and lamb tissue, suggesting that scar development is related to collagen cross-linking or arrangement. This study indicates that healing in the scarless fetal wound is representative of the normal fetal growth pattern, rather than a "response" to burn injury.