S-nitrosoglutathione Reductase-Mediated nitric oxide affects axillary buds outgrowth of solanum lycopersicum L. by regulating auxin and cytokinin signaling.
S-nitrosoglutathione Reductase-Mediated nitric oxide affects axillary buds outgrowth of solanum lycopersicum L. by regulating auxin and cytokinin signaling.
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DOI：
10.1093/pcp/pcab002
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发表时间：
2021-01
影响因子：
4.9
通讯作者：
Yanyan Yan;Q. Shi;B. Gong
中科院分区：
文献类型：
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作者：
Yanyan Yan;Q. Shi;B. Gong
生长素和细胞分裂素是两种重要的植物激素，它们在植物腋芽生长过程中起着重要的调节作用。一氧化氮及其调节因子S-亚硝基谷胱甘肽还原酶（GSNOR）如何参与生长素和细胞分裂素信号转导调控腋芽生长发育至今仍是一个谜。从生理生化和遗传学的角度阐述了GSNOR在番茄腋芽生长发育中的作用。GSNOR负调节NO稳态。抑制GSNOR的表达可通过抑制FZY在顶芽和腋芽中的表达促进腋芽的生长。同时，在GSNOR抑制的植株中，AUX 1和PIN 1在顶芽中表达下调，而在腋芽中表达上调。因此，减少IAA积累的GSNOR抑制植物的顶芽和腋芽。GSNOR介导的NO和生长素的变化影响细胞分裂素的合成、转运和信号转导。GSNOR抑制植株腋芽中生长素与细胞分裂素的比例降低，导致芽休眠解除。我们还发现，最初的NO信号是由硝酸还原酶（NR）催化硝酸盐作为底物产生的。NR介导的NO通过Cys-10的S-亚硝基化降低GSNOR活性，进而诱导NO爆发，从而发挥上述作用促进腋芽生长。总之，GSNOR介导的NO通过改变番茄植株生长素和细胞分裂素的稳态和信号传导在控制腋芽生长中发挥重要作用。
Auxin and cytokinin are two kinds of important phytohormones that mediate outgrowth of axillary buds in plants. How nitric oxide and its regulator of S-nitrosoglutathione reductase (GSNOR) taking part in auxin and cytokinin signaling for controlling axillary buds outgrowth remains elusive. We explained roles of GSNOR during tomato axillary buds outgrowth by physiological, biochemical and genetic approach. GSNOR negatively regulated NO homeostasis. Suppression of GSNOR promoted axillary buds outgrowth via inhibiting the expression of FZY in both apical and axillary buds. Meanwhile, AUX1 and PIN1 were down-regulated in apical buds but up-regulated in axillary buds in GSNOR-suppressed plants. Thus, reduced IAA accumulation was shown in both apical buds and axillary buds of GSNOR-suppressed plants. GSNOR-mediated changes of NO and auxin affected cytokinin biosynthesis, transport, and signaling. And a decreased ratio of auxin: cytokinin was shown in axillary buds of GSNOR-suppressed plants, leading to buds dormancy breaking. We also found that the original NO signaling was generated by nitrate reductase (NR) catalyzing nitrate as substrate. NR-mediated NO reduced the GSNOR activity through S-nitrosylation of Cys-10, then induced a further NO burst, which played the above roles to promote axillary buds outgrowth. Together, GSNOR-mediated NO played important roles in controlling axillary buds outgrowth via altering the homeostasis and signaling of auxin and cytokinin in tomato plants.