Screening of agonistic activities against four nuclear receptors in wastewater treatment plants in Japan using a yeast two-hybrid assay
Screening of agonistic activities against four nuclear receptors in wastewater treatment plants in Japan using a yeast two-hybrid assay
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DOI：
10.1016/s1001-0742(10)60383-x
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发表时间：
2011-01-01
影响因子：
6.9
通讯作者：
Ike, Michihiko
中科院分区：
文献类型：
--
作者：
Inoue, Daisuke;Nakama, Koki;Ike, Michihiko
为了评估城市废水中多种核受体（NR），尤其是非甾体 NR 的潜在内分泌干扰作用，我们使用酵母双杂交测定法检测了日本城市污水处理厂（WWTP）未经处理和处理过的废水对四种 NR（雌激素受体 α、甲状腺激素受体 α、视黄酸受体 α 和类维生素 A X 受体 α）的激动活性。对七个污水处理厂进水和出水的调查表明，在进水中始终检测到针对甾体和非甾体NRs的激动活性，并且部分残留在出水中。对采用传统活性污泥、拟缺氧-好氧、缺氧-好氧和厌氧-缺氧-好氧工艺的四个污水处理厂的进一步研究表明，减少对四种NR的拮抗活性的能力因处理工艺而异。这些结果表明，日本的城市废水通常含有内分泌干扰化学物质，这些化学物质对甾体和非甾体NRs产生激动作用，并且其中一些化学物质被释放到自然水生环境中。尽管酵母检测结果表明，污水处理厂废水中非甾体 NR 激动剂的测量水平不太可能引起任何生物效应，但仍需要进一步研究来详细评估其可能的风险。
To assess the potential endocrine disruptive effects through multiple nuclear receptors (NRs), especially non-steroidal NRs, in municipal wastewater, we examined the agonistic activities on four NRs (estrogen receptor alpha, thyroid hormone receptor alpha, retinoic acid receptor alpha and retinoid X receptor alpha) of untreated and treated wastewater from municipal wastewater treatment plants (WWTPs) in Japan using a yeast two-hybrid assay. Investigation of the influent and effluent of seven WWTPs revealed that agonistic activities against steroidal and non-steroidal NRs were always detected in the influents and partially remained in the effluents. Further investigation of four WWTPs employing conventional activated sludge, pseudo-anoxic-oxic, anoxic-oxic and anaerobic-anoxic-oxic processes revealed that the ability to reduce the agonistic activity against each of the four NRs varies depending on the treatment process. These results indicated that municipal wastewater in Japan commonly contains endocrine disrupting chemicals that exert agonistic activities on steroidal and non-steroidal NRs, and that some of these chemicals are released into the natural aquatic environment. Although the results obtained in yeast assays suggested that measured levels of non-steroidal NR agonists in the effluent of WWTPs were not likely to cause any biological effect, further study is required to assess their possible risks in detail.