Secretory IgA as a diagnostic tool for Pseudomonas aeruginosa respiratory colonization
Secretory IgA as a diagnostic tool for Pseudomonas aeruginosa respiratory colonization
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DOI：
10.1016/j.jcf.2012.07.001
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发表时间：
2013-01-01
影响因子：
5.2
通讯作者：
Hoiby, Niels
中科院分区：
文献类型：
--
作者：
Aanaes, Kasper;Johansen, Helle Krogh;Hoiby, Niels
背景：铜绿假单胞菌鼻窦炎可能是囊性纤维化（CF）患者间歇性肺定植的焦点。鼻窦炎可引起鼻分泌物和唾液中对抗铜绿假单胞菌的IgA水平升高。方法：120例慢性感染、间歇性定植或肺部无铜绿假单胞菌的CF患者参与了这项横断面研究。采用ELISA法检测鼻分泌物、唾液和血清中抗铜绿假单胞菌和海藻酸盐的IgA和IgG。结果：间歇定植的患者鼻分泌物和唾液中的IgA水平明显高于肺部无铜绿假单胞菌的患者，提示铜绿假单胞菌鼻窦炎可能先于间歇定植和肺部慢性感染。结论：鼻分泌物和唾液中针对铜绿假单胞菌的特异性IgA有助于区分慢性感染、间歇性定植和肺部无铜绿假单胞菌的患者。IgA ELISA的诊断价值有待于前瞻性研究。(C) 2012年欧洲囊性纤维化学会。Elsevier B.V.版权所有。
Background: Pseudomonas aeruginosa sinusitis may be the focus for intermittent lung colonization in patients with cystic fibrosis (CF). The sinusitis may induce elevated IgA levels in nasal secretion and saliva against P. aeruginosa.Methods: 120 CF patients chronically infected, intermittently colonized or without P. aeruginosa in the lungs participated in this cross-sectional study. IgA and IgG against P. aeruginosa sonicate and alginate were measured in nasal secretions, saliva, and in serum by ELISA.Results: The intermittently colonized patients had significantly higher IgA levels in nasal secretions and saliva than those without P. aeruginosa in the lungs, indicating that P. aeruginosa sinusitis may precede intermittent colonization and chronic infection of the lungs.Conclusions: Specific IgA against P. aeruginosa in nasal secretions and saliva can contribute to differentiation between patients chronically infected, intermittently colonized, and without P. aeruginosa in the lungs. The diagnostic value of the IgA ELISA awaits a prospective study. (C) 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.