Sequencing two Tyr::CreERT2 transgenic mouse lines
Sequencing two Tyr::CreERT2 transgenic mouse lines
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DOI：
10.1111/pcmr.12842
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发表时间：
2019-11-19
影响因子：
4.3
通讯作者：
Larue, Lionel
中科院分区：
文献类型：
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作者：
Aktary, Zackie;Corvelo, Andre;Larue, Lionel
Cre/loxP系统是一个强大的工具，它允许在各种生物环境中研究特定基因的作用。Tyr::CreERT2系统允许在他莫昔芬治疗后黑素细胞谱系中Cre酶的靶向表达和活性，从而提供特定靶基因表达的空间和时间控制。已经产生了两种独立的转基因小鼠模型，每一种都含有Tyr::CreERT2转基因，并被广泛用于研究黑素细胞转化。在本研究中，我们对两种Tyr::CreERT2小鼠模型的基因组DNA进行了全基因组测序（WGS），并确定了它们在C57BL/6基因组中的整合位点。基于这些结果，我们设计了PCR引物来准确、高效地对转基因小鼠进行基因分型。最后，我们讨论了每种转基因小鼠模型的一些优点。
The Cre/loxP system is a powerful tool that has allowed the study of the effects of specific genes of interest in various biological settings. The Tyr::CreERT2 system allows for the targeted expression and activity of the Cre enzyme in the melanocyte lineage following treatment with tamoxifen, thus providing spatial and temporal control of the expression of specific target genes. Two independent transgenic mouse models, each containing a Tyr::CreERT2 transgene, have been generated and are widely used to study melanocyte transformation. In this study, we performed whole genome sequencing ( WGS) on genomic DNA from the two Tyr::CreERT2 mouse models and identified their sites of integration in the C57BL/6 genome. Based on these results, we designed PCR primers to accurately, and efficiently, genotype transgenic mice. Finally, we discussed some of the advantages of each transgenic mouse model.