Markers of surface mucous cell type human gastric cancer cells: Galactose oxidase‐Schiff reactive mucins, monoclonal antibody SH‐9 reactive mucins and cathepsin E
Markers of surface mucous cell type human gastric cancer cells: Galactose oxidase‐Schiff reactive mucins, monoclonal antibody SH‐9 reactive mucins and cathepsin E
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人胃癌细胞表面粘液细胞标记物：半乳糖氧化酶-希夫反应性粘蛋白、单克隆抗体SH-9反应性粘蛋白和组织蛋白酶E
DOI：
10.1111/j.1440-1827.1993.tb01163.x
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发表时间：
1993
影响因子：
2.2
通讯作者：
K. Imaida
中科院分区：
文献类型：
--
作者：
M. Tatematsu;H. Iwata;M. Ichinose;N. Kakei;S. Tsukada;K. Miki;S. Imal;K. Imaida
胃癌细胞的细胞分化允许通过粘蛋白组织化学和胃蛋白酶原（Pg）免疫组织化学将细胞类型分为表面粘液细胞、幽门腺细胞、肠吸收细胞和杯状细胞类型。先前已通过半乳糖氧化酶-希夫（GOS）反应检测到每种组织学类型胃癌的表面粘液细胞分化，尽管在所有情况下并不总是阳性。正常胃粘膜表面粘液细胞的粘液颗粒对SH-9（抗CA 125抗原分子片段的单克隆抗体）显示出强烈的反应性。组织蛋白酶E也在表面粘液细胞的胞质中表达，在十二指肠绒毛的吸收细胞中弱表达，偶尔在幽门腺细胞中表达。因此，研究了SH-9反应性粘蛋白和组织蛋白酶E的表达，作为区分203例原发性胃癌中胃癌细胞类型的可能的额外标志物。SH-9反应性粘蛋白选择性地存在于表面粘液细胞型的GOS阳性癌细胞和/或未通过mocin组织化学分类的癌细胞中。因此，后者的细胞被归类为表面粘液细胞类别。组织蛋白酶E主要见于GOS阳性的表面粘液细胞型癌细胞，偶尔见于肠吸收型和幽门腺细胞型癌细胞。在所研究的203例原发性胃癌中，分别在145例（71.4%）、151例（74.4%）和144例（70.9%）中发现半乳糖氧化酶-希夫、SH-9和组织蛋白酶E反应或阳性癌细胞。
Cellular differentiation of gastric cancer cells allows the classification of cell type into surface mucous cell, pyloric gland cell, intestinal absorptive cell and goblet cell types by mucin histochemistry and pepsinogen (Pg) immunohistochemistry. Surface mucous cell differentiation of gastric cancers of each histologic type has previously been detected by the galactose oxidase‐Schiff (GOS) reaction although this is not always positive in all cases. Mucus granules of surface mucous cells of normal gastric mucosa show an intense reactivity for SH‐9 (monoclonal antibody against CA125‐bearing antigenic molecule fragments). Cathepsin E is also expressed in the cytoplasm of surface mucous cells, weakly in absorptive cells of duodenal villi and occasionally in pyloric gland cells. Expression of SH‐9 reactive mucin and of cathepsin E were therefore investigated as possible additional markers to distinguish between the gastric cancer cell type in 203 primary stomach cancers. SH‐9 reactive mucin was found selectively in GOS positive cancer cells of surface mucous cell type and/or cancer cells unclassified by mocin histochemistry. These latter cells were therefore classified into the surface mucous cell category. Cathepsin E was found mainly in cancer cells of the GOS positive surface mucous cell type and occasionally, in intestinal absorptive and pyloric gland cell types. Galactose oxidase‐Schiff, SH‐9 and cathepsin E reactive or positive cancer cells were found in 145 (71.4%), 151 (74.4%) and 144 (70.9%), respectively, of the 203 primary stomach cancers investigated.
胃癌中的胃蛋白酶原 I 和 II：一项免疫组织化学研究。
DOI：
--
发表时间：
1985
期刊：
Applied pathology
影响因子：
--
作者：
Stemmermann,GN;Samloff,IM;Hayashi,T
通讯作者：
Hayashi,T
使用凝集素-辣根过氧化物酶缀合物和半乳糖氧化酶-希夫序列对啮齿动物和人类气管腺分泌糖蛋白中的糖残基进行光学显微镜组织化学检测。
DOI：
10.1177/31.3.6186733
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Schulte,BA;Spicer,SS
通讯作者：
Spicer,SS
胃癌的中心凹细胞成分。
影响因子：
3.3
作者：
Fiocca,R;Villani,L;Tenti,P;Cornaggia,M;Finzi,G;Riva,C;Capella,C;Bara,J;Samloff,IM;Solcia,E
通讯作者：
Solcia,E