Interaction between transcription elongation factors and mRNA 3′-end formation at the Saccharomyces cerevisiae GAL10-GAL7 locus
Interaction between transcription elongation factors and mRNA 3′-end formation at the Saccharomyces cerevisiae GAL10-GAL7 locus
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DOI：
10.1074/jbc.m411108200
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发表时间：
2005-01-14
影响因子：
4.8
通讯作者：
Winston, F
中科院分区：
文献类型：
--
作者：
Kaplan, CD;Holland, MJ;Winston, F
Spt6 是一种保守的转录因子，在延伸过程中与 RNA 聚合酶 II (pol II) 结合。 Spt6 对于酿酒酵母的生存至关重要，并在 pol II 转录过程中调节染色质结构。在这里，我们提出证据表明，损害 Spt6、第二个延伸因子、Spt4 和 pol II 的突变可以影响 GAL10 的 3 3' 末端形成。额外的分析表明，Spt6 是因子 Ctr9（Paf1 复合体的成员）与 GAL10 和 GAL7 的共转录关联所必需的，并且 Ctr9 与 GAL10 染色质 3' 的关联受 GAL10 聚腺苷酸化信号的调节。总体而言，这些结果为转录延伸因子 Spt6 与体内 3' 末端形成之间的联系提供了新的证据。
Spt6 is a conserved transcription factor that associates with RNA polymerase II (pol II) during elongation. Spt6 is essential for viability in Saccharomyces cerevisiae and regulates chromatin structure during pol II transcription. Here we present evidence that mutations that impair Spt6, a second elongation factor, Spt4, and pol II can affect 3 3'-end formation at GAL10. Additional analysis suggests that Spt6 is required for cotranscriptional association of the factor Ctr9, a member of the Paf1 complex, with GAL10 and GAL7, and that Ctr9 association with chromatin 3' of GAL10 is regulated by the GAL10 polyadenylation signal. Overall, these results provide new evidence for a connection between the transcription elongation factor Spt6 and 3'-end formation in vivo.