3T3-L1 ADIPOCYTES PROMOTE THE GROWTH OF MAMMARY EPITHELIUM
3T3-L1 ADIPOCYTES PROMOTE THE GROWTH OF MAMMARY EPITHELIUM
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DOI：
10.1016/0014-4827(84)90361-6
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发表时间：
1984-01-01
影响因子：
3.7
通讯作者：
STOCKDALE, FE
中科院分区：
文献类型：
--
作者：
LEVINE, JF;STOCKDALE, FE
小鼠乳腺上皮生长与体内主要是脂肪细胞的造口有关。为探讨脂肪细胞对乳腺上皮细胞的促生长作用，利用3T3-L1细胞系建立了乳腺上皮细胞与脂肪细胞共培养体系。当培养达到融合和生长停止时，这些细胞进行脂肪细胞分化：将中期怀孕的小鼠乳腺上皮细胞种植在经致死照射的3T3-L1脂肪细胞、未分化的3T3-L1细胞、3T3-C2成纤维细胞(3T3细胞的亚克隆，不经历脂肪细胞分化)或组织培养塑料上。脂肪细胞饲养层上的乳腺上皮集落比3T3-C2细胞上的集落大2倍，比组织培养塑料上的集落大4倍。乳腺细胞氚胸腺嘧啶核苷掺入和标记指数在脂肪细胞上显著高于其他饲养层或塑料。将乳腺上皮细胞接种于3T3-L1细胞来源的贴壁材料(细胞外基质)上培养5天后，乳腺细胞数量增加6倍，而在3T3-L1脂肪细胞来源的塑料材料上种植的细胞数量比在非条件培养基中生长的细胞数量增加4倍。乳腺上皮与脂肪细胞的相互作用明显促进了乳腺上皮的增殖，细胞外成分可能参与了这一作用。
Murine mammary epithelium grows in association with predominantly adipocyte stoma in vivo. To investigate potential growth-promoting effects of adipocytes on mammary epithelium, a co-culture system of mammary epithelium and adipocytes was developed by taking advantage of the 3T3-L1 cell line. These cells undergo adipocyte differentiation when the culture reaches confluence and growth ceases: Mid-pregnant murine mammary epithelium was plated on lethally irradiated feeder layers of 3T3-L1 adipocytes, undifferentiated 3T3-L1 cells, 3T3-C2 fibroblasts (a subclone of 3T3 cells that does not undergo adipocyte differentiation), or tissue culture plastic. Mammary epithelial colony size on adipocyte feeder layers was 2-fold larger than colonies on 3T3-C2 cells and 4-fold larger than colonies on tissue culture plastic. Measurement of tritiated thymidine [3H]TdR incorporation and labeling index in mammary cells was significantly higher on adipocytes than on other feeder layers or plastic. There was a 6-fold increase in mammary cell number after 5 days in culture when mammary epithelium was plated on substrate-attached material (extracellular matrix) derived from 3T3-L1 cells and a 4-fold increase in cell number when plated on plastic in conditioned medium derived from 3T3-L1 adipocytes compared with growth on plastic in unconditioned medium. Interaction of mammary epithelium with adipocytes apparently results in a marked increase in proliferation of mammary epithelium; extracellular components may mediate this effect.