Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization.
Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization.
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DOI：
10.2217/fmb-2016-0002
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发表时间：
2016-03
影响因子：
3.1
通讯作者：
Qingqiang Xu;Ming-mei Cao;Hongyuan Song;Shenglin Chen;X. Qian;P. Zhao;H. Ren;Hailin Tang;Yan Wang;You-heng Wei;Yongzhe Zhu;Z. Qi
中科院分区：
文献类型：
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作者：
Qingqiang Xu;Ming-mei Cao;Hongyuan Song;Shenglin Chen;X. Qian;P. Zhao;H. Ren;Hailin Tang;Yan Wang;You-heng Wei;Yongzhe Zhu;Z. Qi
目的探讨乙型脑炎病毒(JEV)进入细胞的详细机制。材料与方法利用靶向细胞膜转运基因的siRNA文库识别介导乙脑病毒进入人神经细胞的关键分子。结果乙脑病毒通过小窝蛋白-1介导的内吞作用进入神经细胞，这种内吞作用依赖于RhoA和rac1对肌动蛋白骨架重构的两步调控：RhoA激活促进小窝蛋白-1的磷酸化，而rac1激活促进小窝蛋白相关病毒的内化。具体地说，病毒附着激活了EGFR-PI3K信号通路，从而导致RhoA激活。结论本工作提供了乙型脑炎病毒进入神经细胞的详细信息和复杂的细胞事件，促进了对乙型脑炎病毒进入神经细胞的更深入的了解。
AIM To investigate the detailed mechanism of Japanese encephalitis virus (JEV) cell entry. MATERIALS & METHODS Utilize a siRNA library targeting cellular membrane trafficking genes to identify key molecules that mediate JEV entry into human neuronal cells. RESULTS JEV enters human neuronal cells by caveolin-1-mediated endocytosis, which depends on a two-step regulation of actin cytoskeleton remodeling triggered by RhoA and Rac1: RhoA activation promoted the phosphorylation of caveolin-1, and then Rac1 activation facilitated caveolin-associated viral internalization. Specifically, virus attachment activates the EGFR-PI3K signaling pathway, thereby leading to RhoA activation. CONCLUSION This work provides a detailed picture of the entry route and intricate cellular events following the entry of JEV into human neuronal cells, and promotes a better understanding of JEV entry.