Molecular evolution of aromatic polyketides and comparative sequence analysis of polyketide ketosynthase and 16S ribosomal DNA genes from various streptomyces species
Molecular evolution of aromatic polyketides and comparative sequence analysis of polyketide ketosynthase and 16S ribosomal DNA genes from various streptomyces species
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DOI：
10.1128/aem.68.9.4472-4479.2002
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发表时间：
2002-09-01
影响因子：
4.4
通讯作者：
Ylihonko, K
中科院分区：
文献类型：
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作者：
Metsä-Ketelä, M;Halo, L;Ylihonko, K
对从土壤中分离的 99 种放线菌中对芳香族聚酮化合物抗生素生物合成途径中必需酮合酶基因的 613 个片段进行了测序。系统发育分析表明，分离物聚集成对应于各种芳香族聚酮化合物类别的进化枝。此外，对 16S 核糖体 DNA (rDNA) 的 γ 可变区的 120-by 片段进行测序以及随后的比较序列分析揭示了酮合酶和 16S rDNA 系统发育树之间的不一致，这强烈表明芳香族聚酮化合物生物合成基因存在水平转移。结果表明，酮合酶树可用于次级代谢物的DNA指纹分析和筛选有趣的芳香族聚酮化合物生物合成基因。此外，酮合酶基因的运动表明，16S rDNA等传统标记分子给出了有关芳香族聚酮化合物生物合成潜力的误导性信息，因此只有直接参与次级代谢产物生物合成的分子才能用于获得有关不同放线菌中抗生素生产的生物多样性的信息。
A 613-by fragment of an essential ketosynthase gene from the biosynthetic pathway of aromatic polyketide antibiotics was sequenced from 99 actinomycetes isolated from soil. Phylogenetic analysis showed that the isolates clustered into clades that correspond to the various classes of aromatic polyketides. Additionally, sequencing of a 120-by fragment from the gamma-variable region of 16S ribosomal DNA (rDNA) and subsequent comparative sequence analysis revealed incongruity between the ketosynthase and 16S rDNA phylogenetic trees, which strongly suggests that there has been horizontal transfer of aromatic polyketide biosynthesis genes. The results show that the ketosynthase tree could be used for DNA fingerprinting of secondary metabolites and for screening interesting aromatic polyketide biosynthesis genes. Furthermore, the movement of the ketosynthase genes suggests that traditional marker molecules like 16S rDNA give misleading information about the biosynthesis potential of aromatic polyketides, and thus only molecules that are directly involved in the biosynthesis of secondary metabolites can be used to gain information about the biodiversity of antibiotic production in different actinomycetes.