ISOLATION OF AUXOTROPHS FROM NEISSERIA MENINGITIDIS.

ISOLATION OF AUXOTROPHS FROM NEISSERIA MENINGITIDIS.
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从脑膜炎奈瑟菌中分离营养缺陷型。

DOI:
10.1111/apm.1965.63.3.435
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发表时间:
1965
期刊:
Acta pathologica et microbiologica Scandinavica
影响因子:
--
通讯作者:
K. Jyssum
K. Jyssum
中科院分区:
--
文献类型:
--
作者:
K. Jyssum

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次芽孢杆菌(17),肺炎双球菌(16)和流感嗜血杆菌(18)。1962年,Catlin和Schloer(3)描述了一种确定的培养基,该培养基满足了使用链霉素耐药标记物进行脑膜炎奈瑟菌转化定量研究的最低要求。然而,在研究脑膜炎奈瑟菌转化的人群中,营养标记似乎并不适用。实际上,没有报道描述了在这种细菌中引入营养泥质酶。这个实验室从事脑膜炎球菌代谢的研究已有一段时间了。鉴于遗传方法作为代谢研究工具的重要性,也开展了关于脑膜炎球菌蛋白融合的研究。在这些研究中,营养标记物被广泛使用。本通讯描述了这些研究中使用的媒介,将一些营养标记物引入脑膜炎奈瑟菌,以及营养不良菌在混合培养中的行为。
Bacillus sublilis (17), Diplococcus pneumoniae (16) and Haemophilus influenzae (18). In 1962 Catlin & Schloer (3) described a defined medium which satisfied minimal requirements for use in quantitative studies of transformation in Neisseria meningilidis with a streptomycin-resistance marker. Nutritional markers, however, do not seem to be in use among those who study transformation in Neisseria meningilidis. Actually, no reports can be found describing the introduction of nutritional marl< ers in this species of bacteria. This laboratory has been engaged for some time in studies on the metabolism of meningococci. With a view to the importance of genetic methods as a tool in metabolic research, investigations were also stal'led concerning meromixis in meningococci. In these studies nutritional markers have been extensively employed. The present communication describes the media used in these studies, the introduction of some nutritional markers into Neisseria meningitidis, and the behaviour of auxotrophs in mixed cultures.