Characterization of 23 CC chemokine genes and analysis of their expression in channel catfish (Ictalurus punctatus)
Characterization of 23 CC chemokine genes and analysis of their expression in channel catfish (Ictalurus punctatus)
复制标题
DOI：
10.1016/j.dci.2005.10.007
复制
发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Liu, Zhanjiang
中科院分区：
文献类型：
--
作者：
Bao, Baolong;Peatman, Eric;Liu, Zhanjiang
趋化因子是一个大家族的趋化细胞因子发挥关键作用的先天性免疫反应。CC趋化因子构成趋化因子的最大亚家族，从哺乳动物物种中鉴定出28种CC趋化因子。然而，CC趋化因子在硬骨鱼中的地位尚未确定。我们以前确定了26鲶鱼CC趋化因子的cDNA从鲶鱼。在这项研究中，我们分离和测序了23个癍点叉尾鮰CC趋化因子基因，总计超过56 kb的基因组序列。通过将产生的基因组序列与先前鉴定的cDNA序列进行比较来确定23个CC趋化因子基因的基因组组织。从16个鲶鱼CC趋化因子基因中鉴定出微卫星，使其可用于基因组作图。结构分析表明CC趋化因子基因的基因组结构保守，这可能有助于建立直系同源。所有已知的鲶鱼CC趋化因子转录本的表达进行了评估，在9个重要的组织。在26个鲶鱼CC趋化因子基因中，14个是普遍表达的，6个在许多组织中广泛表达，而6个是高度组织特异性的。(c)2005爱思唯尔有限公司保留所有权利。
Chemokines are a large family of chemotactic cytokines playing crucial roles in the innate immune response. CC chemokines constitute the largest subfamily of chemokines, with 28 CC chemokines identified from mammalian species. However, the status of CC chemokines in teleosts is yet to be determined. We previously identified 26 catfish CC chemokine cDNAs from catfish. In this study, we isolated and sequenced 23 channel catfish CC chemokine genes amounting to a total of over 56 kb of genomic sequences. Genomic organization of the 23 CC chemokine genes was determined by comparing the generated genomic sequences with the previously identified cDNA sequences. Microsatellites were identified from 16 catfish CC chemokine genes allowing them to be utilized for genome mapping. Structural analysis indicated conservation of genomic organization of CC chemokine genes, which may facilitate the establishment of orthologies. Expression of all known catfish CC chemokine transcripts was assessed in nine important tissues. Of the 26 catfish CC chemokine genes, 14 were universally expressed, six were widely expressed in many tissues, while six were highly tissue- specific. (c) 2005 Elsevier Ltd. All rights reserved.