Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon ( Acipenser schrenckii )
Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon ( Acipenser schrenckii )
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DOI：
10.11648/j.avs.20180604.11
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发表时间：
2018-10
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影响因子：
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通讯作者：
Xiujuan Zhang;Jing-e Ma;Linmiao Li;Haiying Jiang;Jinping Chen
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文献类型：
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作者：
Xiujuan Zhang;Jing-e Ma;Linmiao Li;Haiying Jiang;Jinping Chen
人工繁殖技术的发展在鲟鱼养殖中无论是物种恢复还是商业化生产都变得越来越重要。在产卵季节收集的高质量精液的冷冻保存技术可以极大地提高繁殖效率，全年可用，特别是淡季使用。但目前史氏鲟精子的超低温保存技术还没有得到有效的发展。本研究首次研究了甲醇、二甲基亚砜（DMSO）、丙二醇（PG）3种冷冻保护剂和新鲜卵黄（Y）对史氏鲟精子活力、受精率和胚胎发育的影响。结果表明，MT组精子在4°C保存72 h后活力仍大于60%，而对照组精子活力急剧下降至4.5%。解冻后精子活力测定结果显示，MT+DMSO组与MT组无显著性差异，但MT组的受精率为42.30±2.57（%），显著高于MT+DMSO组（P<0.05）。同时，我们还发现添加Y对解冻后精子活力没有显著的正向影响。有趣的是，尽管结果显示MT+DMSO组和MT组对解冻后精子活力的影响相似，但MT+DMSO组的孵化率高于任何其他测试组，包括MT组。同时，我们也发现PG作为冷冻保护剂不适合于史氏鲟精子的冷冻保存。研究结果为进一步优化史氏鲟人工繁殖技术提供了重要依据。
Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon ( Acipenser schrenckii ) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.