Construction and delivery of tissue-engineered human retinal pigment epithelial cell sheets, using magnetite nanoparticles and magnetic force
Construction and delivery of tissue-engineered human retinal pigment epithelial cell sheets, using magnetite nanoparticles and magnetic force
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DOI：
10.1089/ten.2005.11.489
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发表时间：
2005-03-01
影响因子：
--
通讯作者：
Honda, H
中科院分区：
文献类型：
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作者：
Ito, A;Hibino, E;Honda, H
脉络膜新生血管(CNV)是年龄相关性黄斑变性(AMD)中最严重的一种，会导致快速视力丧失。应用组织工程技术移植培养的视网膜色素上皮(RPE)细胞片是治疗CNV的一种可能方法。在本研究中，我们研究了使用磁性纳米颗粒和磁力在体外构建和传递RPE细胞片的可能性。在ARPE-19人RPE细胞中加入表面带正电荷的磁铁矿阳离子脂质体(MCL)，每细胞磁铁矿浓度为25或50pg，细胞占MCL的40%~55%。将磁性标记的ARPE-19细胞(8×103个/mm(2)，相当于相对于培养区域的融合浓度的10倍[4 mm(2)])种植到超低贴壁平板中，并在井下放置磁铁(4000G)。磁性标记的ARPE-19细胞在培养24小时后形成大约15层的细胞片。当磁铁被移除时，将薄片从平板底部分离出来，然后收获并使用磁铁转移到组织培养皿中。随后，将细胞薄片贴在培养皿上，观察细胞在薄片上生长的情况。这一新的方法被称为“基于磁力的组织工程”(MAG-TE)，是治疗CNV的一种可能的方法。
Choroidal neovascularization (CNV) is the most severe form of age-related macular degeneration (AMD), which causes rapid visual loss. Transplantation of cultured retinal pigment epithelium (RPE) cell sheet by tissue engineering is a possible approach to the treatment of CNV. In the present study, we investigated the possibility of using magnetite nanoparticles and magnetic force to construct and deliver RPE cell sheets in vitro. When magnetite cationic liposomes (MCLs), having a positive charge at the surface, were added to ARPE-19 human RPE cells at a concentration of 25 or 50 pg of magnetite per cell, the cells took up 40 to 55% of the MCLs. The magnetically labeled ARPE-19 cells (8 x 103 cells/mm(2), which corresponds to 10-fold the confluent concentration against the culture area [4 mm(2)]) were seeded into an ultra-low-attachment plate and a magnet (4000 G) was placed under the well. The magnetically labeled ARPE-19 cells formed an approximately 15-layered cell sheet after a 24 h of culture. When the magnet was removed, the sheets were detached from the bottom of the plate and then harvested and transferred to a tissue culture dish, using a magnet. Subsequently, the cell sheets were attached onto the dish, and the cells growing on the sheets were observed. This novel methodology, termed "magnetic force-based tissue engineering" (Mag- TE), is a possible approach for CNV treatment.