Psychological stress-induced accelerated colonic transit in rats involves hypothalamic corticotropin-releasing factor.

Psychological stress-induced accelerated colonic transit in rats involves hypothalamic corticotropin-releasing factor.
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DOI：
10.1016/0016-5085(93)91006-4
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发表时间：
1993-03
期刊：
Gastroenterology
影响因子：
29.4
通讯作者：
H. Mönnikes;B. Schmidt;Y. Taché
H. Mönnikes;B. Schmidt;Y. Taché
中科院分区：
医学1区
文献类型：
--
作者：
H. Mönnikes;B. Schmidt;Y. Taché

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背景：脑促肾上腺皮质激素释放因子（CRF）参与应激引起的加速结肠转运。在清醒喂养的大鼠中研究 CRF 刺激结肠转运的脑部作用部位。方法：将双侧引导套管长期植入下丘脑 (PVN) 的室旁核 (PVN) 或中央杏仁核进行肽显微注射，并将导管插入近端结肠以测量结肠转运。结果：将 CRF (0.6 nmol/大鼠) 注射到 PVN 中，使结肠转运时间缩短 84%，并刺激粪便颗粒排出20 倍，而 CRF 注射到 PVN 或中央杏仁核之外的部位则没有效果。氯茚明和硝酸甲酯阿托品可阻止 CRF 刺激作用，但溴苄铵则不能阻止 CRF 刺激作用。站在小立方体上避免接触水的压力使结肠传输时间减少了 75%，粪便排出量增加了 7 倍。将 CRF 拮抗剂 α-helical-CRF9-14 双侧显微注射到 PVN 中，消除了结肠对应激的反应。 CRF拮抗剂对非应激大鼠的基础结肠转运时间没有影响。结论：心理应激诱导的进食大鼠结肠运动功能的刺激涉及PVN中的CRF通路。
Background:Brain corticotropin-releasing factor (CRF) is involved in stress-induced accelerated colonic transit. Brain sites of action of CRF to stimulate colonie transit were investigated in conscious fed rats.Methods:Bilateral guide cannulae were chronically implanted into the paraventricular nucleus of the hypothalamus (PVN) or central amygdala for peptide microinjection and a catheter into the proximal colon to measure colonic transit.Results:CRF (0.6 nmol/rat) injected into the PVN reduced colonic transit time by 84% and stimulated fecal pellet output 20-fold, whereas CRF injected into sites outside of the PVN or the central amygdala had no effect. CRF stimulatory action was prevented by chlorisondamine, and atropine methyl nitrate but not by bretylium. The stress of avoiding water by standing on a small cube reduced colonic transit time by 75% and increased fecal output by 7-fold. Bilateral microinjection of CRF antagonist, α-helical-CRF9–14, into the PVN abolished the colonic response to stress. The CRF antagonist had no effect on basal colonic transit time in nonstressed rats.Conclusions:Psychological stress-induced stimulation of colonic motor function in fed rats involves CRF pathways in the PVN.