The brain link protein-1 (BRAL1):: cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain
The brain link protein-1 (BRAL1):: cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain
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DOI：
10.1006/bbrc.2000.3583
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发表时间：
2000-10-05
影响因子：
3.1
通讯作者：
Ninomiya, Y
中科院分区：
文献类型：
--
作者：
Hirakawa, S;Oohashi, T;Ninomiya, Y
本文报道了一种主要在大脑中表达的新型人脑连接蛋白-1 （BRAL1）的分子克隆和基因表达分析。人脑连接蛋白-1的开放阅读框编码一个340个氨基酸的多肽，包含3个蛋白质模块，免疫球蛋白样折叠和蛋白多糖串联重复1和2结构域，估计质量为38 kDa。脑连接蛋白-1 mRNA仅存在于脑中。当在小鼠发育过程中分析时，它仅在成年大脑中被检测到。脑连接蛋白-1和多种蛋白聚糖的mrna在脑中的同时表达模式表明，脑连接蛋白-1可能具有稳定透明质酸和灯盏蛋白结合的功能。人类BRAL1基因含有7个外显子，长度约为6 kb。整个免疫球蛋白样折叠由一个外显子编码，蛋白多糖串联重复I和2结构域分别由一个外显子和两个外显子编码。人脑连接蛋白-1的氨基酸序列与人类软骨连接蛋白-1 （CRTL1）有45%的一致性，此前报道的CRTL1是稳定软骨中聚集蛋白和透明质酸聚集的连接蛋白。这些结果表明，脑连接蛋白-1可能与软骨连接蛋白-1具有不同的功能，并发挥特定的作用，特别是在成人大脑中。(C) 2000年学术出版社。
We report here molecular cloning and expression analysis of the gene for a novel human brain link protein-1 (BRAL1) which is predominantly expressed in brain. The predicted open reading frame of human brain link protein-1 encoded a polypeptide of 340 amino acids containing three protein modules, the immunoglobulin-like fold and proteoglycan tandem repeat 1 and 2 domains, with an estimated mass of 38 kDa. The brain link protein-1 mRNA was exclusively present in brain. When analyzed during mouse development, it was detected solely in the adult brain. Concomitant expression pattern of mRNAs for brain link protein-1 and various lectican proteoglycans in brain suggests a possibility that brain link protein-1 functions to stabilize the binding between hyaluronan and brevican, The human BRAL1 gene contained 7 exons and spanned similar to 6 kb. The entire immunoglobulin-like fold was encoded by a single exon and the proteoglycan tandem repeat I and 2 domains were encoded by a single and two exons, respectively. The deduced amino acid sequence of human brain link protein-1 exhibited 45% identity with human cartilage link protein-1 (CRTL1), previously reported as link protein to stabilize aggregates of aggrecan and hyaluronan in cartilage. These results suggest that brain link protein-1 may have distinct function from cartilage link protein-1 and play specific roles, especially in the adult brain. (C) 2000 Academic Press.