Intraventricular transplantation of embryonic stem cell-derived neural stem cells in intracerebral hemorrhage rats
Intraventricular transplantation of embryonic stem cell-derived neural stem cells in intracerebral hemorrhage rats
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DOI：
10.1179/016164104225014049
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发表时间：
2004-04-01
影响因子：
1.9
通讯作者：
Sakaki, T
中科院分区：
文献类型：
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作者：
Nonaka, M;Yoshikawa, M;Sakaki, T
本研究旨在探讨胚胎干细胞移植治疗脑出血的可行性。以胶原酶诱导的脑出血大鼠为模型动物。用全反式维甲酸（ATRA）诱导小鼠ES细胞分化为nestin阳性神经干细胞。胶原酶输注后7天，将ATRA处理的ES细胞（10（5））移植到出血对侧半球的侧脑室中。移植后28天，在所有接受移植的10只大鼠的脑血肿腔周围观察到ES源性神经元和星形胶质细胞。在侧脑室室管膜下区发现了Craft源性神经元，呈细胞结节状。虽然接受移植的10只大鼠中有1只表现出来源于ES细胞的星形胶质细胞的不受控制的生长，但ATRA处理的ES细胞的脑室内移植是向ICH部位递送神经元谱系定向祖细胞的有效递送系统。
In the present study, we attempted to explore cell transplantation therapy for intracerebral hemorrhage (ICH) using embryonic stem (ES) cells. Collagenase-induced ICH rats were used as model animals. Mouse ES cells were differentiated into nestin-positive neural stem cells in vitro by alltrans retinoic acid (ATRA). ATRA-treated ES cells (10(5)) were transplanted into the literal ventricle in the hemisphere contralateral to the hemorrhage 7 days after collagenase infusion. Twenty-eight days after transplantation, ES-derived neurons and astrocytes were observed around the hematoma cavities of the brain in all of the ten rats receiving grafts. Craft-derived neurons were found in the subependymal area of the literal ventricle as cellular nodules. Although one of the ten rats receiving grafts showed uncontrolled growth of astroglia derived from the ES cells, intraventricular transplantation of ATRA-treated ES cells is an effective delivery system of neuronal lineage-committed progenitor cells toward the site of ICH.