Fungicidal activity of secondary metabolites from Annulohypoxylon sp. against the rice blast fungus, Pyricularia oryzae
Fungicidal activity of secondary metabolites from Annulohypoxylon sp. against the rice blast fungus, Pyricularia oryzae
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DOI：
10.1007/s10327-020-00936-0
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发表时间：
2020-06
影响因子：
1.2
通讯作者：
Yoshiya Moriguchi;J. Kihara;M. Ueno
中科院分区：
文献类型：
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作者：
Yoshiya Moriguchi;J. Kihara;M. Ueno
稻瘟病是一种由稻瘟病菌引起的毁灭性病害，给水稻生产造成重大损失，但它不断进化出对杀菌剂的抗药性。因此，需要鉴定天然化合物来开发新的药剂来控制真菌病原体。在接种 P 分生孢子前 24 小时，将分离株 S712（2009 年从岛根县稻田采集的多孔菌科成员）的培养滤液 (S712-ECF) 的乙酸乙酯提取物喷洒在稻叶上。米霉。 S712-ECF 显着抑制叶片上稻瘟病斑的形成。对S712的内转录间隔区(ITS)-5.8S rDNA序列分析表明，其与真菌Annulohypoxylon物种具有高度相似性。 S712-ECF显着抑制P.分生孢子的体外萌发。 oryzae 以剂量依赖性方式对 P. 具有杀菌作用。米霉。 P 的生长抑制区。在 S712-ECF 的薄层色谱分析中检测到了米曲霉。 S712-ECF 中最有效的化合物具有热稳定性，分子量 < 3000 Da。因此，分离株 S712 的次生代谢物具有开发新的抗疟原虫杀菌剂的潜力。米霉。
Rice blast, a devastating disease caused by the fungusPyricularia oryzae, causes major losses to rice production, but it continuously evolves resistance against fungicides. Therefore, natural compounds need to be identified to develop new agents to control fungal pathogens. An ethyl acetate extract of a culture filtrate (S712-ECF) from isolate S712, a member of Polyporaceae collected from a paddy field in Shimane Prefecture in 2009, was sprayed on rice leaves 24 h before inoculation with conidia ofP. oryzae. S712-ECF significantly suppressed blast lesion formation on leaves. Analysis of the internal transcribed spacer (ITS)-5.8S rDNA sequence of S712 showed that it shared high similarities with species of the fungusAnnulohypoxylon. S712-ECF significantly inhibited in vitro conidial germination ofP. oryzaein a dose-dependent manner and had a fungicidal effect againstP. oryzae. Growth inhibition zones ofP. oryzaewere detected in thin layer chromatography assays of S712-ECF. The most effective compound in S712-ECF was heat-stable with a molecular weight < 3000 Da. Secondary metabolites of isolate S712 thus have potential for developing a new fungicidal agent againstP. oryzae.