The role of RANTES in nasal polyposis
The role of RANTES in nasal polyposis
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DOI：
10.1177/194589240501900103
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Maune, S
中科院分区：
文献类型：
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作者：
Meyer, JE;Bartels, J;Maune, S
背景：嗜酸性粒细胞的特征性浸润是鼻息肉(NPs)的特征。一些研究表明，CC趋化因子家族的成员可能参与了这一过程。RANTES(受激活调节，正常T细胞表达和分泌)是CC趋化因子家族成员，主要对嗜酸性粒细胞和T淋巴细胞具有趋化活性。因此，RANTES是嗜酸性粒细胞和T淋巴细胞重新聚集到鼻腔的有趣靶点。据报道，组织嗜酸性粒细胞增多症的程度与疾病症状的严重程度、下呼吸道的侵犯程度以及NPs复发的可能性有关。因此，我们假设高数量的嗜酸性粒细胞与高水平的RANTES相关，相关的特应性疾病改变了这种相关性。方法：从NP匀浆中提取总RNA，逆转录，采用半定量逆转录聚合酶链式反应和Northern印迹分析RANTES mRNA的表达。组织学研究将NPs分为嗜酸性粒细胞和低嗜酸性粒细胞两组。用RANTES特异的酶联免疫吸附试验检测匀浆中RANTES蛋白的浓度。结果：本研究清楚地表明RANTES在NPs中表达和分泌。组织嗜酸性粒细胞增多组RANTES基因表达和蛋白产量显著高于无组织嗜酸性粒细胞增多组。乙酰水杨酸不耐受与慢性增生性鼻窦炎/鼻窦炎的单独符合还显著增加了NPs中RANTES的含量。结论：RANTES增加导致组织嗜酸性粒细胞增多。相关的乙酰水杨酸不耐受似乎增加了NPs中RANTES的数量，这可能部分解释了这些患者更严重的临床过程。因此，RANTES似乎在动员嗜酸性粒细胞进入局部炎症组织中起着重要作用。
Background: Characteristic infiltrates of eosinophils are a hallmark of nasal polyps (NPs). Several studies suggest that members of the CC chemokine family may be involved in this process. RANTES (regulated on activation, normal t-cell-expressed and secreted) is a member of the CC chemokine family with chemotactic activity on mainly eosinophils and T lymphocytes. Thus, RANTES is an interesting target for the recruitment of eosinophils and T lymphocytes into the nose. The degree of the tissue eosinophilia has been reported to correlate with the severeness of the symptomatology of the disease and the extension on the lower respiratory tract, as well as with the probability of the recurrence of NPs. Therefore, we hypothesized that high numbers of eosinophils correlate with high levels of RANTES and that associated atopic diseases modify this correlation.Methods: Total RNA was extracted from NP homogenates, reverse transcribed and RANTES mRNA expression analyzed using semiquantitative reverse transcription polymerase chain reaction and Northern blot analysis. Histological studies divided NPs in an eosinophilic and low eosinophilic group. Additionally, RANTES protein concentration was measured in homogenates by a RANTES-specific enzyme-linked immunosorbent assay.Results: This study has clearly shown that RANTES is expressed and secreted in NPs. The group with a high tissue eosinophilia had a significant higher RANTES gene expression and protein production than NPs without tissue eosinophilia. The isolated coincidence of acetylsalicyl acid intolerance with chronic hyperplastic sinusitis/NP additionally increased significantly the RANTES amounts in NPs.Conclusion: Increased RANTES leads to increased tissue eosinophilia. Associated acetylsalicylic acid intolerance seems to enhance the amount of RANTES in NPs and might explain in part the more severe clinical course in those patients. Thus, RANTES appears to play an important role in mobilization of eosinophils into the local inflamed tissue.